Bhunia A K, Westbrook D G, Story R, Johnson M G
Department of Food Science, University of Arkansas, Fayetteville 72704, USA.
J Clin Microbiol. 1995 Dec;33(12):3349-51. doi: 10.1128/jcm.33.12.3349-3351.1995.
Murine hybridoma cells, designated Ped-2E9, when stored up to 60 days at -196 degrees C or up to 48 days at -80 degrees C, gave results equivalent to those for freshly grown murine hybridoma cells in an in vitro pathogenicity assay of Listeria species. Thus, laboratories do not need to have their own tissue culture facilities to maintain the hybridoma cells for the assay described.
名为Ped-2E9的小鼠杂交瘤细胞,在-196℃下储存长达60天或在-80℃下储存长达48天时,在李斯特菌属的体外致病性试验中给出的结果与新鲜培养的小鼠杂交瘤细胞相当。因此,实验室无需拥有自己的组织培养设施来维持用于所述试验的杂交瘤细胞。