Cardeñosa N, Riera C, Cortés P, March F, Muñoz C, Portús M, Prats G
Departament de Microbiologia, Hospital de la Santa Creu i Sant Pau, Universitat Autònoma de Barcelona, Spain.
Parasite Immunol. 1995 Oct;17(10):509-16. doi: 10.1111/j.1365-3024.1995.tb00881.x.
Humoral immune responses were studied in 53 sera from 18 patients with visceral leishmaniasis by immunoblot analysis. Sera from visceral leishmaniasis patients recognized antigens with molecular weights ranging from < 14 kDa to more than 100 kDa. Bands ranging between 49 and < 14 kDa were the most specific. The 40, 33 and 17 kDa antigens were recognized by 90%, 79% and 79% of the patients sera, respectively. Sera from one patient with Chagas' disease identified 8 of 11 antigens of the specific region. Treatment with periodate eliminated the cross-reaction in three of these antigens (40, 29, 26 kDa). The study of serial sera collected from the different patients showed a decrease in intensity or dissappearance in some of the diagnostic bands, particularly the 17 kDa band. The band of 17 kDa seems to be useful to study the clinical evolution, for post-treatment control and also for epidemiologic purposes. (It has been identified in 7% of control sera from endemic areas.) Immunoblot could be a valuable tool in the diagnosis of visceral leishmaniasis, being more sensitive and specific than other serologic tests.
通过免疫印迹分析，对18例内脏利什曼病患者的53份血清中的体液免疫反应进行了研究。内脏利什曼病患者的血清识别分子量范围从小于14 kDa到超过100 kDa的抗原。49 kDa至小于14 kDa之间的条带最为特异。40、33和17 kDa抗原分别被90%、79%和79%的患者血清识别。1例恰加斯病患者的血清识别出特定区域11种抗原中的8种。高碘酸盐处理消除了其中3种抗原（40、29、26 kDa）的交叉反应。对不同患者采集的系列血清进行的研究显示，一些诊断条带的强度降低或消失，尤其是17 kDa条带。17 kDa条带似乎有助于研究临床病程、治疗后监测以及流行病学目的。（在流行地区7%的对照血清中已识别出该条带。）免疫印迹可能是诊断内脏利什曼病的一种有价值的工具，比其他血清学检测更敏感、特异。
