Rolland L, Belkaid M, Seye A, Schneider P, Gentilini M
Laboratoire de Parasitologie, Faculté de Médecine Pitié-Salpêtrière, Paris, France.
Parasite. 1995 Mar;2(1):13-21. doi: 10.1051/parasite/1995021013.
Leishmania promastigotes polypeptides are analyzed by immunoblotting with sera from patients infected with different Leishmania species and presenting visceral or cutaneous infections. These sera recognize Leishmania polypeptides in several molecular masses. The major findings of this study are as follow. 1) The Leishmania 94 kDa antigen, which is specifically recognized by all sera from L. infantum-infected patients with visceral infection, is recognized by some sera from L. infantum-infected patients presenting cutaneous infection. 2) All patients with cutaneous infections due to L. tropica, L. amazonensis, or L. guyanensis do not develop anti-94 kDa antibodies, whatever the Leishmania species used as antigens. 3) Difference in electrophoretic mobilities is seen between the 94 kDa antigen identified by sera from Leishmania infantum-infected patients, and the antigen both recognized by the Concavalin A lectin and a rabbit antiserum raised against deglycosylated Promastigote Surface Protease.
利用感染不同利什曼原虫物种并患有内脏或皮肤感染的患者血清，通过免疫印迹法分析利什曼原虫前鞭毛体多肽。这些血清可识别几种分子量的利什曼原虫多肽。本研究的主要发现如下：1）94 kDa利什曼原虫抗原可被所有来自内脏感染的婴儿利什曼原虫感染患者的血清特异性识别，也可被一些来自患有皮肤感染的婴儿利什曼原虫感染患者的血清识别。2）所有因热带利什曼原虫、亚马逊利什曼原虫或圭亚那利什曼原虫导致皮肤感染的患者，无论用作抗原的利什曼原虫物种如何，均未产生抗94 kDa抗体。3）婴儿利什曼原虫感染患者血清识别的94 kDa抗原与伴刀豆球蛋白A凝集素和针对去糖基化前鞭毛体表面蛋白酶产生的兔抗血清均识别的抗原之间，存在电泳迁移率差异。
