Rothwell L, Gramzinski R A, Rose M E, Kaiser P
Institute for Animal Health, Compton, Nr. Newbury, Berkshire, UK.
Parasite Immunol. 1995 Oct;17(10):525-33. doi: 10.1111/j.1365-3024.1995.tb00883.x.
The effect of infection and subsequent challenge with Eimeria maxima on the populations of lymphocytes in the small intestine of Light Sussex chickens was assessed by immunohistochemistry. T cells were characterized for CD3, CD4, CD8, TCR1 (gamma delta heterodimer) or TCR2 (alpha beta 1 heterodimer) markers, and B cells for the expression of IgM, IgA and IgG. After a primary inoculum there were, in both the epithelium and the lamina propria, two distinct increases in the numbers of T lymphocytes. The first peaked on days 3-5 and the second, greater influx, on day 11 after infection. CD4+ and CD8+ cells were represented in both peaks but, whereas CD4+ cells were found almost exclusively in the lamina propria, CD8+ cells were present in both sites. The area staining positive for CD8+ cells was somewhat greater than the value obtained for CD4+ cells. In the epithelium there was an early, small increase in TCR1(+)-staining, followed by a larger rise to the second peak, at which time there was also an increase in the lamina propria. Staining for TCR2+ cells followed the same pattern with a reversed distribution between epithelium and lamina propria. Changes after challenge were minimal and confined to the epithelium. The most notable changes in the expression of immunoglobulins were, in the lamina propria, a biphasic increase in the amount of IgM(+)-staining in the course of primary infection (corresponding approximately to that of the T cells), and in IgA+ cells shortly after challenge.
通过免疫组织化学评估了感染和随后用巨型艾美耳球虫攻击对浅花苏塞克斯鸡小肠淋巴细胞群体的影响。T细胞通过CD3、CD4、CD8、TCR1（γδ异二聚体）或TCR2（αβ1异二聚体）标记物进行表征，B细胞通过IgM、IgA和IgG的表达进行表征。初次接种后，在上皮和固有层中，T淋巴细胞数量出现了两次明显增加。第一次在感染后第3 - 5天达到峰值，第二次更大的流入在感染后第11天出现。两个峰值中均有CD4 +和CD8 +细胞，但CD4 +细胞几乎仅存在于固有层中，而CD8 +细胞在两个部位均有存在。CD8 +细胞的阳性染色面积略大于CD4 +细胞的值。在上皮中，TCR1(+)染色早期有小幅增加，随后升至第二个峰值时大幅上升，此时固有层中也有增加。TCR2 +细胞的染色遵循相同模式，上皮和固有层之间的分布相反。攻击后的变化很小，仅限于上皮。固有层中免疫球蛋白表达最显著的变化是，在初次感染过程中IgM(+)染色量呈双相增加（大致与T细胞的增加相对应），以及在攻击后不久IgA +细胞增加。
