Pouliquen H, Algoet M, Buchet V, Le Bris H
Laboratoire de pharmacie et toxicologie, Ecole Nationale Veterinaire, Nantes, France.
Vet Hum Toxicol. 1995 Dec;37(6):527-9.
Some land-based marine fish-farms situated on the Atlantic coastline of France use high volumes of underground sea water. Studies of the available quantities and movements of this underground resource became necessary, using fluorescent dyes such as fluorescein. As fluorescein may reach reared fish, it became important to assess its toxicity to fish. Acute fluorescein toxicity to turbot (Scophthalmus maximus) was investigated by exposing fish to different fluorescein concentrations (0.5, 0.7, 0.9, 1.1 and 1.3 milligrams) at 14.0 C for 24, 48 or 96 h. The lethal concentration inducing 50% fish mortality (LC50) was 997.1 +/- 11.4 mg/l (mean +/- standard deviation) after a 24, 48 or 96 h exposure. Toxicity affected the central nervous system. Early postmortem findings were a brown-green coloring of some tissues and encephalon congestion. The fluorescein LC50 was much higher than the recommended concentration in field applications (1 mg/l), indicating that fluorescein toxicity to turbot will not be expected when used at the recommended concentration.
法国大西洋沿岸的一些陆地海洋养鱼场使用大量的地下海水。因此，有必要利用荧光素等荧光染料对这种地下资源的可用量和流动情况进行研究。由于荧光素可能会接触到养殖鱼类，评估其对鱼类的毒性就变得很重要。通过在14.0℃下将鱼暴露于不同的荧光素浓度（0.5、0.7、0.9、1.1和1.3毫克）24、48或96小时，研究了荧光素对大菱鲆（Scophthalmus maximus）的急性毒性。在暴露24、48或96小时后，导致50%鱼类死亡的致死浓度（LC50）为997.1 +/- 11.4毫克/升（平均值 +/- 标准差）。毒性影响中枢神经系统。死后早期发现一些组织呈棕绿色，脑部充血。荧光素的LC50远高于现场应用中的推荐浓度（1毫克/升），这表明在推荐浓度下使用时，荧光素对大菱鲆不会产生毒性。
