Yoshida T, Sakane N, Wakabayashi Y, Umekawa T, Kondo M
First Department of Internal Medicine, Kyoto Prefectural University of Medicine, Japan.
Int J Obes Relat Metab Disord. 1995 Oct;19(10):717-22.
To investigate the hypothesis that anti-obesity action of bofu-tsusho-san (TJ-62) works via activating the brown adipose tissue thermogenesis and inhibiting the phosphodiesterase activity.
MSG obese mice and lean controls were fed a diet including 1.4% or 4.7% TJ-62 of weight of food for 8 weeks. Another group of MSG obese mice were fed with 1-ephedrine (1-E) + d-pseudoephedrine (d-PE) of equivalent amounts as contained in TJ-62 (4.7%) for 8 weeks. Yet another group of MSG obese mice were further supplemented with Glycyrrhizae Radix (GR) extract + Forsythiae Fructus (FF) extract + Schizonepetae Spica (SS) extract (that inhibited phosphodiesterase activity) of amounts contained in TJ-62 (4.7%) for 8 weeks.
The following were measured: The concentration of ephedrine and its congeners in TJ-62; the inhibitory effect of TJ-62 on phosphodiesterase activity; body weight; food intake; retroperitoneal white adipose tissue (RWAT) weight; interscapular brown adipose tissue (IBAT) weight; mitochondrial protein content in IBAT; cytochrome c oxidase activity in IBAT; guanosine-5'-diphosphate (GDP) binding in IBAT mitochondria.
One gram of TJ-62 contained 3.33 mg of 1-E and 0.73 mg of d-PE. One mg of TJ-62 was equivalent to 2.5 mg of caffeine in the inhibitory effect on phosphodiesterase activity. After feeding with TJ-62, GDP binding was significantly increased in a dose dependent manner. Body weight and RWAT weight decreased in both MSG obese mice and lean controls. Food intake was not changed by TJ-62. Feeding with 1-E + d-PE produced responses of about 70% of those of TJ-62. These responses were, furthermore, enhanced by the addition of the three extracts to the levels that were similar to those produced by TJ-62.
Bofu-tsusho-san (TJ-62) works via activating the BAT thermogenesis and inhibiting the phosphodiesterase activity in mice.
研究防风通圣散（TJ - 62）的抗肥胖作用是否通过激活棕色脂肪组织产热和抑制磷酸二酯酶活性来实现。
将MSG肥胖小鼠和瘦小鼠作为对照，喂养含食物重量1.4%或4.7%的TJ - 62的饮食8周。另一组MSG肥胖小鼠喂养与TJ - 62（4.7%）等量的1 - 麻黄碱（1 - E）+ d - 伪麻黄碱（d - PE）8周。还有一组MSG肥胖小鼠进一步补充TJ - 62（4.7%）中所含剂量的甘草根（GR）提取物+连翘果实（FF）提取物+荆芥穗（SS）提取物（抑制磷酸二酯酶活性）8周。
测量以下指标：TJ - 62中麻黄碱及其同系物的浓度；TJ - 62对磷酸二酯酶活性的抑制作用；体重；食物摄入量；腹膜后白色脂肪组织（RWAT）重量；肩胛间棕色脂肪组织（IBAT）重量；IBAT中的线粒体蛋白含量；IBAT中的细胞色素c氧化酶活性；IBAT线粒体中的鸟苷 - 5'-二磷酸（GDP）结合。
1克TJ - 62含有3.33毫克1 - E和0.73毫克d - PE。1毫克TJ - 62对磷酸二酯酶活性的抑制作用相当于2.5毫克咖啡因。喂养TJ - 62后，GDP结合呈剂量依赖性显著增加。MSG肥胖小鼠和瘦小鼠的体重及RWAT重量均下降。TJ - 62对食物摄入量无影响。喂养1 - E + d - PE产生的反应约为TJ - 62的70%。此外，添加三种提取物后这些反应增强至与TJ - 62产生的反应水平相似。
防风通圣散（TJ - 62）通过激活小鼠棕色脂肪组织产热和抑制磷酸二酯酶活性发挥作用。
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