Boone D R, Liu Y, Zhao Z J, Balkwill D L, Drake G R, Stevens T O, Aldrich H C
Department of Environmental Science and Engineering, Oregon Graduate Institute of Science & Technology, Portland 97291-1000, USA.
Int J Syst Bacteriol. 1995 Jul;45(3):441-8. doi: 10.1099/00207713-45-3-441.
Bacillus infernus sp. nov. was isolated from ca. 2,700 m below the land surface in the Taylorsville Triassic Basin in Virginia. B. infernus was a strict anaerobe that grew on formate or lactate with Fe(III), MnO2, trimethylamine oxide, or nitrate (reduced to nitrite) as an electron acceptor, and it also grew fermentatively on glucose. Type strain TH-23 and five reference strains were gram-positive rods that were thermophilic (growth occurred at 61 degrees C), halotolerant (good growth occurred in the presence of Na+ concentrations up to 0.6 M), and very slightly alkaliphilic (good growth occurred at pH 7.3 to 7.8). A phylogenetic analysis of its 16S rRNA indicated that B. infernus should be classified as a new species of the genus Bacillus. B. infernus is the only strictly anaerobic species in the genus Bacillus.
新种地下芽孢杆菌（Bacillus infernus sp. nov.）是从弗吉尼亚州泰勒维尔三叠纪盆地地表以下约2700米处分离得到的。地下芽孢杆菌是一种严格厌氧菌，以甲酸盐或乳酸盐为碳源，以Fe(III)、MnO₂、氧化三甲胺或硝酸盐（还原为亚硝酸盐）作为电子受体进行生长，并且它也能在葡萄糖上进行发酵生长。模式菌株TH-23和五个参考菌株均为革兰氏阳性杆菌，具有嗜热特性（在61℃时生长）、耐盐特性（在Na⁺浓度高达0.6 M的情况下生长良好）以及极轻微的嗜碱特性（在pH 7.3至7.8时生长良好）。对其16S rRNA的系统发育分析表明，地下芽孢杆菌应被归类为芽孢杆菌属的一个新物种。地下芽孢杆菌是芽孢杆菌属中唯一的严格厌氧物种。
