Larsen V H, Freudendal-Pedersen A, Fogh-Andersen N
Department of Anaesthesiology and Clinical Chemistry, Herlev Hospital, University of Copenhagen, Denmark.
Acta Anaesthesiol Scand Suppl. 1995;107:53-5. doi: 10.1111/j.1399-6576.1995.tb04331.x.
Patent Blue V (PBV) is a blue dye solution which is used to visualize lymphatic vessels for surgical procedures. There has been some conflicting reports about the influence of this dye solution on pulse oximetry and haemoximetry, why we decided to: 1) produce a spectrum of PBV in plasma, 2) measure absorbances of full blood before and after addition of PBV and 3) to record the effect on the pulse oximeter and haemoximeter of an intradermal injection of PBV to a patient. Computer analysis of the blood gas measurements were carried out by the Oxygen Status Algorithm (OSA). The spectrum of PBV demonstrated a peak absorption at 640 nm and correspondingly the absorbances of the haemoximeter increased most significantly at 622 and 636 nm. These changes invalidated the determination of haemoglobin pigments, and the results should not be used. Computer analysis interpreted the measurements as a shift of the haemoglobin oxygen binding curve.
专利蓝V（PBV）是一种蓝色染料溶液，用于在外科手术中使淋巴管可视化。关于这种染料溶液对脉搏血氧饱和度测定法和血液氧合测定法的影响，一直存在一些相互矛盾的报告，因此我们决定：1）生成血浆中PBV的光谱，2）测量添加PBV前后全血的吸光度，3）记录向患者皮内注射PBV对脉搏血氧仪和血液氧合仪的影响。通过氧状态算法（OSA）对血气测量结果进行计算机分析。PBV的光谱在640nm处显示出一个吸收峰，相应地，血液氧合仪的吸光度在622和636nm处增加最为显著。这些变化使血红蛋白色素的测定无效，因此不应使用这些结果。计算机分析将这些测量结果解释为血红蛋白氧结合曲线的偏移。
