Zeilicoff R, Ameigeiras B, Ojeda E, Isla Rodríguez R, Grünbaum S, Genero M, Cappelletti C, Tielli G, Roatta R, Koch O
Hospital Municipal Ramos Mejía, Departamento de Hepatología, Buenos Aires, Argentina.
Acta Gastroenterol Latinoam. 1995;25(3):163-70.
We have investigated the presence of genomic and replicative RNA strands of hepatitis C virus in liver and serum. Eleven patients with proven chronic hepatitis C, received Interferon a2a 4,5 MU, three times a week during six months. RT-PCR was used with sense primer to detect the replicative strand and an antisense primer to identify genomic strand. Before treatment, genomic strands were present in liver and serum of all patients. Replicative strands were present in liver and serum in five and six cases, respectively. Seven out of eleven responded to treatment. In responders, genomic strands were absent in liver of 3 cases (43%) and replicative strands in liver of 4 (57%). In plasma genomic and replicative strands were absent in 5 (71%) and 7 (100%), respectively. In all non responders, genomic strands in liver and plasma remained present. Replicative strands in liver and plasma were present in 100% and 25%, respectively. Knodell score improved in 5 out of 7 responders and remained unchanged in 3 out of 4 non responders. In 2 out of 4 responders with genomic and replicative strands in liver, Knodell score remained unchanged or worse. In all non responders, genomic and replicative strands in liver were present and Knodell score remained unchanged or worse. Genomic and replicative strands in plasma tended to be negative after treatment in responders. Genomic strands in plasma remained present in non responders. Conversely, genomic and replicative strands in liver were present in all non responders. It seems to exist a relationship between genomic and replicative strands in liver and the same or worse Knodell score. After a follow up, it will be possible to determined whether responders who still present viral RNA in liver would be prone to a relapse.
我们研究了丙型肝炎病毒基因组和复制性RNA链在肝脏和血清中的存在情况。11例确诊为慢性丙型肝炎的患者，接受了450万单位的α2a干扰素治疗，每周3次，持续6个月。使用正义引物通过逆转录聚合酶链反应（RT-PCR）检测复制链，用反义引物鉴定基因组链。治疗前，所有患者的肝脏和血清中均存在基因组链。复制链分别在5例患者的肝脏和6例患者的血清中存在。11例患者中有7例对治疗有反应。在有反应者中，3例（43%）肝脏中不存在基因组链，4例（57%）肝脏中不存在复制链。在血浆中，5例（71%）不存在基因组链，7例（100%）不存在复制链。在所有无反应者中，肝脏和血浆中仍存在基因组链。肝脏和血浆中的复制链分别有100%和25%存在。7例有反应者中有5例Knodell评分改善，4例无反应者中有3例评分保持不变。在4例肝脏中存在基因组和复制链的有反应者中，有2例Knodell评分保持不变或恶化。在所有无反应者中，肝脏中存在基因组和复制链，Knodell评分保持不变或恶化。有反应者治疗后血浆中的基因组和复制链倾向于呈阴性。无反应者血浆中仍存在基因组链。相反，所有无反应者的肝脏中均存在基因组和复制链。肝脏中的基因组和复制链与相同或更差的Knodell评分之间似乎存在关联。经过随访，将有可能确定肝脏中仍存在病毒RNA的有反应者是否易于复发。
