慢性丙型肝炎患者肝脏和血清中丙型肝炎病毒RNA复制中间体的检测
Detection of replicative intermediates of hepatitis C viral RNA in liver and serum of patients with chronic hepatitis C.
作者信息
Fong T L, Shindo M, Feinstone S M, Hoofnagle J H, Di Bisceglie A M
机构信息
Liver Diseases Section, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892.
出版信息
J Clin Invest. 1991 Sep;88(3):1058-60. doi: 10.1172/JCI115368.
Abstract
The hepatitis C virus is a positive stranded hepatotropic RNA virus. We describe a method of detecting positive and negative strands of hepatitis C viral RNA using the polymerase chain reaction. We tested serum and liver tissue from nine patients with chronic hepatitis C. The positive RNA strand of HCV was detected in the sera and livers of all nine, the negative strand was detected in the livers of eight (89%), and in the sera of five (55%). Titers of both strands of HCV RNA were determined by serial endpoint dilutions. The amount of the negative strand in the serum and liver was usually 10-100 times less than the positive strand. Predigestion of serum with ribonucleases did not alter the detection of the negative strand. This suggests that the negative strand found in the serum may be protected from digestion by being associated with virions.
摘要
丙型肝炎病毒是一种正链嗜肝RNA病毒。我们描述了一种使用聚合酶链反应检测丙型肝炎病毒RNA正负链的方法。我们检测了9例慢性丙型肝炎患者的血清和肝组织。所有9例患者的血清和肝脏中均检测到HCV的正链RNA，8例（89%）患者的肝脏中检测到负链RNA，5例（55%）患者的血清中检测到负链RNA。通过连续终点稀释法测定HCV RNA两条链的滴度。血清和肝脏中负链的量通常比正链少10 - 100倍。用核糖核酸酶对血清进行预消化不会改变负链的检测结果。这表明血清中发现的负链可能通过与病毒粒子结合而免受消化。
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