Fillola G, Muller C, Bousquet R, Fontanilles A M, Laharrague P, Corberand J X
Laboratoire d'Hématologie, CHU Rangueil, Toulouse, France.
J Immunol Methods. 1996 Mar 28;190(1):127-31. doi: 10.1016/0022-1759(95)00269-3.
In order to isolate bone marrow plasma cells from patients presenting with multiple myeloma or monoclonal gammopathy of undetermined significance, we developed a method for purifying these cells by negative selection using monoclonal antibodies and immunomagnetic beads. The results presented here were obtained from 75 procedures. Purity was extremely variable (2-100%) and was dependent on the percentage of plasma cells in the original bone marrow sample with a 10% cut-off, beyond which purity was over 96% in all cases. The mean yield was about 20%. The cells collected were viable and suitable for immunophenotyping, semi-quantitative studies of oncoproteins, and PCR.
