PRP31基因编码一种酿酒酵母中前体mRNA剪接所需的新型蛋白质。
The PRP31 gene encodes a novel protein required for pre-mRNA splicing in Saccharomyces cerevisiae.
作者信息
Weidenhammer E M, Singh M, Ruiz-Noriega M, Woolford J L
机构信息
Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213 USA.
出版信息
Nucleic Acids Res. 1996 Mar 15;24(6):1164-70. doi: 10.1093/nar/24.6.1164.
Abstract
The pre-mRNA splicing factor Prp31p was identified in a screen of temperature-sensitive yeast strains for those exhibiting a splicing defect upon shift to the non- permissive temperature. The wild-type PRP31 gene was cloned and shown to be essential for cell viability. The PRP31 gene is predicted to encode a 60 kDa polypeptide. No similarities with other known splicing factors or motifs indicative of protein-protein or RNA-protein interaction domains are discernible in the predicted amino acid sequence. A PRP31 allele bearing a triple repeat of the hemagglutinin epitope has been generated. The tagged protein is functional in vivo and a single polypeptide species of the predicted size was detected by Western analysis with proteins from yeast cell extracts. Functional Prp31p is required for the processing of pre-mRNA species both in vivo and in vitro, indicating that the protein is directly involved in the splicing pathway.
摘要
在前体mRNA剪接因子Prp31p的研究中，通过对温度敏感型酵母菌株进行筛选，找出那些在转移至非允许温度时表现出剪接缺陷的菌株。野生型PRP31基因被克隆出来，并证明其对细胞活力至关重要。PRP31基因预计编码一个60 kDa的多肽。在预测的氨基酸序列中，未发现与其他已知剪接因子或指示蛋白质-蛋白质或RNA-蛋白质相互作用结构域的基序有相似之处。已产生一个带有血凝素表位三重重复的PRP31等位基因。标记的蛋白质在体内具有功能，通过对酵母细胞提取物中的蛋白质进行Western分析，检测到了预测大小的单一多肽物种。功能性Prp31p在体内和体外对前体mRNA的加工都是必需的，这表明该蛋白质直接参与剪接途径。
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本文引用的文献
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Six novel genes necessary for pre-mRNA splicing in Saccharomyces cerevisiae.酿酒酵母中前体mRNA剪接所需的六个新基因。
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