Hu H, Tomasiewicz H, Magnuson T, Rutishauser U
Department of Genetics, Case Western Reserve University, Cleveland, Ohio 44106, USA.
Neuron. 1996 Apr;16(4):735-43. doi: 10.1016/s0896-6273(00)80094-x.
Transplantation studies have been used to show that tangential migration of olfactory bulb interneuron precursors is retarded in NCAM-mutant mice, and that this defect reflects loss of NCAM polysialic acid (PSA). In contrast, radial migration of cells within the bulb did not require PSA. Reciprocal transplantations between wild-type and mutant mice have revealed that the mutation affects the in vivo migration environment in the subventricular zone, and not movement of individual cells. However, in vitro migration of the cells into a PSA-negative collagen matrix environment was also PSA dependent. The surprisingly similar results obtained in the in vivo and in vitro environments is consistent with the observation that migration of subventricular cells occurs as streams of closely apposed cells in which the PSA-positive cells appear to serve as their own migration substrate.
移植研究已被用于表明，嗅球中间神经元前体的切向迁移在NCAM突变小鼠中受到阻碍，并且这种缺陷反映了NCAM多唾液酸（PSA）的缺失。相比之下，嗅球内细胞的径向迁移并不需要PSA。野生型和突变型小鼠之间的相互移植表明，该突变影响脑室下区的体内迁移环境，而非单个细胞的移动。然而，细胞在体外向PSA阴性的胶原基质环境中的迁移也依赖于PSA。在体内和体外环境中获得的惊人相似结果与以下观察结果一致，即脑室下细胞的迁移是以紧密相邻的细胞流形式发生的，其中PSA阳性细胞似乎充当了自身的迁移底物。
