Benković J, Furedi-Milhofer H, Hlady V, Cvorisćec D, Stavljenić-Rukavina A
Klinicki zavod za laboratorijsku dijagnostiku, Klinicki bolnicki centar Zagreb, Croatia.
Eur J Clin Chem Clin Biochem. 1995 Oct;33(10):705-10. doi: 10.1515/cclm.1995.33.10.705.
The effect of Tamm-Horsfall protein isolated from urine of healthy subjects on calcium oxalate precipitation was studied in model systems of precipitation. The study was performed using following conditions: concentrations of calcium chloride 10 mmol/l, sodium chloride 150 mmol/l, oxalic acid 300 mumol/l; pH 6.0, and temperature 310 K. The concentration of Tamm-Horsfall protein varied between 1-10 mg/l. The kinetics of calcium oxalate precipitation was observed by measuring the number and volume of particles in the suspension, and the precipitate composition by an optic microscope. In all the studied systems, the precipitate morphology corresponded to pure calcium oxalate monohydrate. Tamm-Horsfall protein was found to inhibit the growth of calcium oxalate monohydrate crystals and stimulate their aggregation in the given experimental conditions. Both effects were enhanced by increase in the concentrations of Tamm-Horsfall protein and were most pronounced at the concentration of Tamm-Horsfall protein of 10 mg/l.
在沉淀模型系统中研究了从健康受试者尿液中分离出的Tamm-Horsfall蛋白对草酸钙沉淀的影响。研究在以下条件下进行：氯化钙浓度10 mmol/l、氯化钠浓度150 mmol/l、草酸浓度300 μmol/l；pH值6.0，温度310 K。Tamm-Horsfall蛋白的浓度在1 - 10 mg/l之间变化。通过测量悬浮液中颗粒的数量和体积来观察草酸钙沉淀的动力学，并通过光学显微镜观察沉淀成分。在所有研究系统中，沉淀形态均对应于纯一水合草酸钙。发现在给定实验条件下，Tamm-Horsfall蛋白可抑制一水合草酸钙晶体的生长并促进其聚集。这两种作用都随着Tamm-Horsfall蛋白浓度的增加而增强，且在Tamm-Horsfall蛋白浓度为10 mg/l时最为明显。
