Garcia C, Viehman G, Hitchcock M, Clark R E
Dermatologic Surgery and Cutaneous Oncology Unit, Duke University Medical Center, Durham, North Carolina 27710, USA.
Dermatol Surg. 1996 Feb;22(2):177-9. doi: 10.1111/j.1524-4725.1996.tb00503.x.
Immunohistochemical stains for CD34 antigen can help in differentiating dermatofibrosarcoma protuberans (DFSP) from other fibrohistiocytic tumors and in demarcating its surgical margins during Mohs surgery. However, variable expression of CD34 antigen in nodular areas can sometimes result in negative staining of tissue sections creating diagnostic and therapeutic dilemmas.
To show the variable expression of CD34 antigen in a DFSP tumor.
Case presentation of DFSP with immunohistochemical staining using the ABC peroxidase macromolecular complex.
Microscopic examination of paraffin-embedded sections showed CD34-negative DFSP tumor cells with positive staining of endothelial cells. On frozen sections at the time of surgery the tumor cells stained strongly positive for CD34.
This case demonstrates the variable expression of CD34 antigen by DFSP tumors and emphasizes the need to interpret with caution the results of these immunostains during Mohs surgery.
CD34抗原的免疫组织化学染色有助于将隆突性皮肤纤维肉瘤（DFSP）与其他纤维组织细胞肿瘤区分开来，并在莫氏手术中划定其手术切缘。然而，结节区域中CD34抗原的表达可变，有时会导致组织切片染色阴性，从而产生诊断和治疗难题。
展示CD34抗原在DFSP肿瘤中的可变表达。
通过使用ABC过氧化物酶大分子复合物进行免疫组织化学染色对DFSP进行病例展示。
石蜡包埋切片的显微镜检查显示，DFSP肿瘤细胞CD34染色阴性，而内皮细胞染色阳性。手术时的冰冻切片显示肿瘤细胞CD34染色强阳性。
本病例证明了DFSP肿瘤中CD34抗原的可变表达，并强调在莫氏手术期间对这些免疫染色结果进行谨慎解读的必要性。
