Echtenacher B, Männel D N, Hültner L
Institut für Pathologie/Tumorimmunologie, Universität Regensburg, Germany.
Nature. 1996 May 2;381(6577):75-7. doi: 10.1038/381075a0.
Mast cells play a detrimental role in IgE-dependent allergic reactions. In contrast, a protective function for mast cells has been proposed on the basis of some worm infection models. No reports exist on the in vivo significance of these cells in bacterial infections. Here we use congenitally mast-cell-deficient W/Wv mice and normal +/+ littermates to analyse the role of mast cells in a model of acute septic peritonitis (caecum ligation and puncture (CLP)). Following CLP, W/Wv mice showed a significantly increased mortality compared to +/+ mice. The selective reconstitution of W/Wv mice with cultured +/+ mast cells substantially protected them from the lethal effects of CLP, whereas an anti-tumor-necrosis-factor (TNF) antibody injected immediately after CLP completely suppressed this protection. Our results reveal a previously unrecognized protective role of mast cells and mast-cell-derived TNF in acute bacterial peritonitis.
肥大细胞在IgE依赖性过敏反应中发挥有害作用。相比之下，基于一些蠕虫感染模型，有人提出肥大细胞具有保护功能。关于这些细胞在细菌感染中的体内意义尚无报道。在此，我们使用先天性肥大细胞缺陷的W/Wv小鼠和正常的+/+同窝小鼠，来分析肥大细胞在急性化脓性腹膜炎（盲肠结扎和穿刺（CLP））模型中的作用。CLP后，与+/+小鼠相比，W/Wv小鼠的死亡率显著增加。用培养的+/+肥大细胞对W/Wv小鼠进行选择性重建，可使其基本免受CLP的致死效应，而CLP后立即注射的抗肿瘤坏死因子（TNF）抗体则完全抑制了这种保护作用。我们的结果揭示了肥大细胞和肥大细胞衍生的TNF在急性细菌性腹膜炎中以前未被认识到的保护作用。
