Kleinkauf H, Von Döhren H
Institute of Biochemistry and Molecular Biology, Technical University Berlin, Germany.
Eur J Biochem. 1996 Mar 1;236(2):335-51. doi: 10.1111/j.1432-1033.1996.00335.x.
This review covers peptide structures originating from the concerted action of enzyme systems without the direct participation of nucleic acids. Biosynthesis proceeds by formation of linear peptidyl intermediates which may be enzymatically modified as well as transformed into specific cyclic structures. The respective enzyme systems are constructed of biosynthetic modules integrated into multienzyme structures. Genetic and DNA-sequence analysis of biosynthetic gene clusters have revealed extensive similarities between prokaryotic and eukaryotic systems, conserved principles of organisation, and a unique mechanism of transport of intermediates during elongation and modification steps involving 4'-phospho-pantetheine. These similarities permit the identification of peptide synthetases and related aminoacyl-ligases and acyl-ligases from sequence data. Similarities to other biosynthetic systems involved in the assembly of polyketide metabolites are discussed.
本综述涵盖了源自酶系统协同作用且无核酸直接参与的肽结构。生物合成通过线性肽基中间体的形成进行，这些中间体可进行酶促修饰并转化为特定的环状结构。相应的酶系统由整合到多酶结构中的生物合成模块构成。生物合成基因簇的遗传和DNA序列分析揭示了原核生物和真核生物系统之间的广泛相似性、保守的组织原则，以及在涉及4'-磷酸泛酰巯基乙胺的延伸和修饰步骤中中间体独特的转运机制。这些相似性使得能够从序列数据中鉴定肽合成酶以及相关的氨酰连接酶和酰基连接酶。文中还讨论了与参与聚酮代谢物组装的其他生物合成系统的相似性。
