Dan P, Nitzan D W, Dagan A, Ginsburg I, Yedgar S
Department of Biochemistry, Hebrew-University-Hadassah Medical School, Jerusalem, Israel.
FEBS Lett. 1996 Mar 25;383(1-2):75-8. doi: 10.1016/0014-5793(96)00227-x.
Phospholipase A2 (PLA2) and H2O2, secreted from activated inflammatory cells, play a central role in the tissue damage occurring in inflammatory processes. However, while exogenous PLA2 alone does not cause cell lysis, it readily does so when acting with H2O2. We have found that H2O2 degrades cell surface proteoglycans, thus rendering the membrane PL accessible to hydrolysis by exogenous PLA2. This novel mechanism introduces a role for cell surface proteoglycans in protection of cells from damage by pro-inflammatory agents, and may assign a central role for the combined action of H2O2 and PLA2 in inflammatory and bacteriocidal processes.
活化的炎症细胞分泌的磷脂酶A2（PLA2）和过氧化氢（H2O2）在炎症过程中发生的组织损伤中起核心作用。然而，虽然外源性PLA2单独不会导致细胞裂解，但它与H2O2一起作用时很容易导致细胞裂解。我们发现H2O2会降解细胞表面蛋白聚糖，从而使膜磷脂易于被外源性PLA2水解。这种新机制揭示了细胞表面蛋白聚糖在保护细胞免受促炎剂损伤中的作用，并可能赋予H2O2和PLA2的联合作用在炎症和杀菌过程中的核心地位。
