Wershil B K, Furuta G T, Wang Z S, Galli S J
Combined Program in Pediatric Gastroenterology and Nutrition, Children's Hospital, Boston, Massachusetts, USA.
Gastroenterology. 1996 May;110(5):1482-90. doi: 10.1053/gast.1996.v110.pm8613053.
BACKGROUND & AIMS: Immunoglobulin (Ig) E-dependent reactions elicit an immediate response and can also result in a late-phase reaction that is characterized by the infiltration of leukocytes. This study assessed whether IgE-dependent late-phase responses can be elicited in the stomach wall of mice and examined the role of mast cells in this reaction.
IgE-dependent gastric inflammation was elicited in genetically mast cell-deficient KitW/KitW-v mice, the congenic normal (+/+) mice, and mast cell-deficient KitW/KitW-v mice that had undergone local and selective reconstitution of gastric mast cell populations.
IgE-dependent gastric reactions were associated with mast cell degranulation and the infiltration of both neutrophils and mononuclear cells in normal mice, but no significant leukocyte infiltration was observed in mast cell-deficient KitW/KitW-v mice. By contrast, in mast cell-reconstituted KitW/KitW-v mice, IgE-dependent reactions were associated with the infiltration of neutrophils and mononuclear cells.
These results show that late-phase reactions can occur during IgE-dependent gastric inflammation in the mouse and that the infiltration of both neutrophils and mononuclear cells that are observed during this reaction are mast cell dependent.
免疫球蛋白（Ig）E 依赖性反应引发即刻反应，也可导致以白细胞浸润为特征的迟发性反应。本研究评估了 IgE 依赖性迟发性反应是否能在小鼠胃壁中引发，并研究了肥大细胞在该反应中的作用。
在基因敲除肥大细胞的 KitW/KitW-v 小鼠、同基因正常（+/+）小鼠以及局部和选择性重建胃肥大细胞群体的肥大细胞缺陷型 KitW/KitW-v 小鼠中引发 IgE 依赖性胃炎症。
在正常小鼠中，IgE 依赖性胃反应与肥大细胞脱颗粒以及中性粒细胞和单核细胞浸润有关，但在肥大细胞缺陷型 KitW/KitW-v 小鼠中未观察到明显的白细胞浸润。相比之下，在肥大细胞重建的 KitW/KitW-v 小鼠中，IgE 依赖性反应与中性粒细胞和单核细胞浸润有关。
这些结果表明，在小鼠 IgE 依赖性胃炎症期间可发生迟发性反应，且该反应中观察到的中性粒细胞和单核细胞浸润均依赖于肥大细胞。
