用针对金黄色葡萄球菌无毒α毒素突变体的抗血清进行被动免疫在小鼠模型中具有保护作用。
Passive immunization with antiserum to a nontoxic alpha-toxin mutant from Staphylococcus aureus is protective in a murine model.
作者信息
Menzies B E, Kernodle D S
机构信息
Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA.
出版信息
Infect Immun. 1996 May;64(5):1839-41. doi: 10.1128/iai.64.5.1839-1841.1996.
Abstract
A nonhemolytic, nonlethal variant of Staphylococcus aureus alpha-toxin constructed via oligonucleotide-directed mutagenesis and containing a single amino acid substitution (H-35 to L) was used to immunize a rabbit. The resulting antiserum was cross-reactive with wild-type alpha-toxin and neutralized its hemolytic activity in vitro. Passive immunization of mice with rabbit antiserum conferred protection against lethal challenge with wild-type alpha-toxin and against acute lethal challenge with a high-alpha-toxin -producing S. aureus strain. H35L alpha-toxin may be useful as a protective immunogen in S. aureus vaccine studies.
摘要
通过寡核苷酸定向诱变构建的、含有单个氨基酸取代（H-35 变为 L）的金黄色葡萄球菌α毒素的非溶血、非致死变体被用于免疫一只兔子。所得抗血清与野生型α毒素交叉反应，并在体外中和其溶血活性。用兔抗血清对小鼠进行被动免疫可使其免受野生型α毒素的致死性攻击以及高α毒素产生的金黄色葡萄球菌菌株的急性致死性攻击。H35Lα毒素可能作为金黄色葡萄球菌疫苗研究中的保护性免疫原有用。
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