Biffl W L, Moore E E, Moore F A, Barnett C C, Silliman C C, Peterson V M
Department of Surgery, Denver General Hospital, CO 80204, USA.
J Leukoc Biol. 1996 Apr;59(4):569-74. doi: 10.1002/jlb.59.4.569.
Interleukin-6 (IL-6) is an integral mediator of the acute phase response to injury and infection; an exaggerated IL-6 response has been associated with adverse clinical events. The precise role of IL-6 is unclear, but it appears capable of modulating the functional repertoire of mature neutrophils (PMNs). Our previous work demonstrated that IL-6 -stimulated PMNs are primed by lower concentrations of platelet-activating factor (PAF) than nonstimulated PMNs. Recently, we have found that IL-6 suppresses PMN apoptosis via a PAF-like mechanism. We hypothesized that IL-6 stimulates PMNs to produce PAF. PMNs isolated from healthy human donors were incubated with IL-6 (0.1-100 ng/ml) at 37 degrees C. Lipid production was measured by use of thin-layer chromatography, and PAF quantitated with a scintillation proximity assay. IL-6 (1 and 10 ng/ml) stimulated PMNs to produce increase quantities of PAF. PAF production was associated with an increase in PMN cytosolic calcium. These data may provide mechanistic insight into IL-6 regulation of PMN-mediated cytotoxicity and the role of PAF in mediating IL-6 effects on PMNs.
白细胞介素-6（IL-6）是损伤和感染急性期反应的重要介质；IL-6反应过度与不良临床事件相关。IL-6的确切作用尚不清楚，但它似乎能够调节成熟中性粒细胞（PMN）的功能谱。我们之前的研究表明，与未受刺激的PMN相比，IL-6刺激的PMN对较低浓度的血小板活化因子（PAF）更为敏感。最近，我们发现IL-6通过一种类似PAF的机制抑制PMN凋亡。我们推测IL-6刺激PMN产生PAF。从健康人类供体分离的PMN在37℃下与IL-6（0.1 - 100 ng/ml）孵育。通过薄层色谱法测量脂质生成，并使用闪烁邻近分析法对PAF进行定量。IL-6（1和10 ng/ml）刺激PMN产生更多量的PAF。PAF的产生与PMN胞质钙的增加有关。这些数据可能为IL-6调节PMN介导的细胞毒性的机制以及PAF在介导IL-6对PMN作用中的作用提供深入了解。
