Dasgupta A, Banerjee P
Department of Pathology, University of New Mexico School of Medicine, Albuqueraue 87106, USA.
Life Sci. 1996;58(15):1285-90. doi: 10.1016/0024-3205(96)00090-2.
We studied lipid profiles of hybrid cells derived from fusion of sympathetic ganglia and neuroblastoma cells (NCB 20, F 11) and also CHO (Chinese hamster ovary) cells. The proportion of saturated to unsaturated fatty acids changed significantly in different phospholipid fractions. We observed 35.4% of total fatty acids as saturated fatty acids in the phosphatidyl ethanolamine (PE) fraction of NCB-20 cell lines while 47.2% of total fatty acids were saturated in the phosphatidyl inositol (PI) fraction. In general, in neuroblastoma cell lines, we observed the lowest proportion of saturated fatty acids in the PE fraction while the other lipid fraction showed a much higher proportion of saturated fatty acids. On the other hand the PE fraction of a non neuronal cell line, CHO, showed 57.4% saturated fatty acids in the PE fraction. This significant difference in saturated to unsaturated fatty acid ratios in different phospholipid head groups may be linked to the different biological functions of those hybrid cells.
我们研究了源自交感神经节与神经母细胞瘤细胞（NCB 20、F 11）以及中国仓鼠卵巢（CHO）细胞融合的杂交细胞的脂质谱。不同磷脂组分中饱和脂肪酸与不饱和脂肪酸的比例发生了显著变化。我们观察到，在NCB - 20细胞系的磷脂酰乙醇胺（PE）组分中，35.4%的总脂肪酸为饱和脂肪酸，而在磷脂酰肌醇（PI）组分中，47.2%的总脂肪酸为饱和脂肪酸。总体而言，在神经母细胞瘤细胞系中，我们观察到PE组分中饱和脂肪酸的比例最低，而其他脂质组分中饱和脂肪酸的比例则高得多。另一方面，非神经细胞系CHO的PE组分中，饱和脂肪酸含量为57.4%。不同磷脂头部基团中饱和脂肪酸与不饱和脂肪酸比例的这一显著差异可能与这些杂交细胞的不同生物学功能有关。
