Gulaya N M, Volkov G L, Klimashevsky V M, Govseeva N N, Melnik A A
A.V. Palladine Institute of Biochemistry, Academy of Sciences, Ukrainian SSR, Kiev.
Neuroscience. 1989;30(1):153-64. doi: 10.1016/0306-4522(89)90361-8.
Phospholipids and cholesterol were found to be the main lipids in mature and immature neuroblastoma cells. The ratios for the total cholesterol/phospholipids in these undifferentiated and differentiated cells were 0.33 and 0.52, respectively. The ratios of 0.45 and 0.62 were obtained with corresponding plasma membrane fractions. Individual fatty acid contents in the loosely bound lipid fraction were higher than in tightly bound lipids. The total levels of saturated fatty acids increased in both of these fractions. While arachidonic acid content significantly decreased, it increased simultaneously (600%) in the free fatty acid fraction during differentiation. The amount of cholesterol esters increased three-fold as a result of maturation. For the first time it was possible to detect, in neuroblastoma cells, several lipids, namely N-acylphosphatidylethanolamine, N-acylethanolamine and semilysobisphosphatidic acid. They all changed during maturation. Total N-acylphosphatidylethanolamine content decreased by 50%, disappearing completely from membrane fractions. N-Acylethanolamine disappeared from the cell as well as from membrane fractions. On the other hand the total cellular content of semilysobisphosphatidic acid increased without any alterations in its membrane content. Functional implications of our investigations are discussed.
磷脂和胆固醇被发现是成熟和未成熟神经母细胞瘤细胞中的主要脂质。这些未分化和分化细胞中总胆固醇与磷脂的比率分别为0.33和0.52。相应质膜部分的比率分别为0.45和0.62。松散结合脂质部分中个别脂肪酸含量高于紧密结合脂质。这两个部分中饱和脂肪酸的总水平均有所增加。虽然花生四烯酸含量显著下降，但在分化过程中其在游离脂肪酸部分中同时增加了600%。由于成熟，胆固醇酯的量增加了三倍。首次在神经母细胞瘤细胞中检测到几种脂质，即N-酰基磷脂酰乙醇胺、N-酰基乙醇胺和半溶血双磷脂酸。它们在成熟过程中均发生了变化。总N-酰基磷脂酰乙醇胺含量下降了50%，在膜部分中完全消失。N-酰基乙醇胺在细胞以及膜部分中均消失。另一方面，半溶血双磷脂酸的总细胞含量增加，而其膜含量没有任何变化。讨论了我们研究的功能意义。
