Nakano T, Kodama H, Honjo T
Department of Medical Chemistry, Faculty of Medicine, Kyoto University, Japan.
Science. 1996 May 3;272(5262):722-4. doi: 10.1126/science.272.5262.722.
During mouse embryogenesis the production of "primitive" erythrocytes (EryP) precedes the production of "definitive" erythrocytes (EryD) in parallel with the transition of the hematopoietic site from the yolk sac to the fetal liver. On a macrophage colony-stimulating factor-deficient stromal cell line OP9, mouse embryonic stem cells were shown to give rise to EryP and EryD sequentially with a time course similar to that seen in murine ontogeny. Studies of the different growth factor requirements and limiting dilution analysis of precursor frequencies indicate that most EryP and EryD probably developed from different precursors by way of distinct differentiation pathways.
在小鼠胚胎发育过程中，“原始”红细胞（EryP）的产生先于“定型”红细胞（EryD），同时造血部位从卵黄囊过渡到胎儿肝脏。在巨噬细胞集落刺激因子缺陷的基质细胞系OP9上，小鼠胚胎干细胞被证明可依次产生EryP和EryD，其时间进程与小鼠个体发育中所见相似。对不同生长因子需求的研究以及前体细胞频率的极限稀释分析表明，大多数EryP和EryD可能通过不同的分化途径从不同的前体细胞发育而来。
