Père M C
Station de Recherches Porcines, Institut National de la Recherche Agronomique, Saint-Gilles, France.
J Anim Sci. 1995 Oct;73(10):2994-9. doi: 10.2527/1995.73102994x.
To study nutrition and metabolism in the fetal pig, a chronic catheterization method was developed that allows blood sampling in arteries and veins, at both the umbilical and uterine sources, in the conscious, unstressed animal. A catheter was inserted in the fetal aorta through a femoral artery, and another one was introduced in the umbilical vein. A catheter was put in a femoral artery of the sow so that its end was in the abdominal aorta. A fourth catheter was placed in a uterine vein draining the fetoplacental unit studied. This procedure was applied to 18 Large White primiparous sows at 99 d of gestation. Blood samples were drawn simultaneously using the four catheters before a meal at 103 d of pregnancy, and glucose, insulin, lactate, and fructose were determinated. Glycemia was 2.5 times higher in the sow than in the fetus. The extraction coefficient of glucose by the fetus amounted to 14% of the umbilical supply. The insulin level in the fetal pig was very low ( < 5 microU/mL). Lactate and fructose seemed to originate from the placenta. Blood lactate was 2.6 times lower in the sow than in the fetus, and its extraction coefficient by the fetus amounted to 8%. Fructose in the fetal blood was 2.3 times higher than that of glucose. Fructose was not utilized by the pig fetus. The present results obtained in the fetal pig are comparable to the conclusions drawn from studies with other species.
为了研究胎猪的营养与代谢，开发了一种慢性插管方法，该方法能够在清醒、无应激的动物身上，从脐部和子宫来源的动脉和静脉采集血液样本。通过股动脉将导管插入胎猪主动脉，另一个导管插入脐静脉。将一个导管放入母猪的股动脉，使其末端位于腹主动脉。第四个导管放置在引流所研究的胎儿 - 胎盘单位的子宫静脉中。该操作应用于18头妊娠99天的大白初产母猪。在妊娠103天喂食前，使用四个导管同时采集血样，并测定葡萄糖、胰岛素、乳酸和果糖。母猪的血糖水平比胎儿高2.5倍。胎儿对葡萄糖的摄取系数相当于脐部供应的14%。胎猪的胰岛素水平非常低（<5微单位/毫升）。乳酸和果糖似乎起源于胎盘。母猪血液中的乳酸比胎儿低2.6倍，胎儿对其摄取系数为8%。胎血中的果糖比葡萄糖高2.3倍。猪胎儿不利用果糖。在胎猪身上获得的目前结果与从其他物种研究得出的结论相当。
