用于检测抗分枝杆菌药物的高通量筛选
High-throughput screen for detecting antimycobacterial agents.
作者信息
Chung G A, Aktar Z, Jackson S, Duncan K
机构信息
Glaxo Research and Development Ltd., Stevenage, Hertfordshire, United Kingdom.
出版信息
Antimicrob Agents Chemother. 1995 Oct;39(10):2235-8. doi: 10.1128/AAC.39.10.2235.
Abstract
A simple, robust assay system which can be used to screen for inhibitors of mycobacterial growth has been developed. A strain of the rapidly growing saprophyte Mycobacterium aurum is used as the test organism. Inhibition of its growth is highly predictive of activity against Mycobacterium tuberculosis, which cannot itself be used in screening because of its growth characteristics and highly infectious nature. The viability of M. aurum in the presence of a test sample is monitored by measuring the uptake of radiolabelled uracil into the cells. In a microtiter plate format, the screen has the potential for testing several thousand samples per day.
摘要
已经开发出一种简单、可靠的检测系统，可用于筛选分枝杆菌生长抑制剂。快速生长的腐生菌金色分枝杆菌菌株用作测试生物体。抑制其生长高度预示着对结核分枝杆菌的活性，结核分枝杆菌由于其生长特性和高度传染性，本身不能用于筛选。通过测量放射性标记的尿嘧啶进入细胞的摄取量来监测金色分枝杆菌在测试样品存在下的活力。以微量滴定板形式进行筛选，每天有潜力测试数千个样品。
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