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编码大肠杆菌中红霉素2'-磷酸转移酶I的红霉素抗性决定子的核苷酸序列及特性

Nucleotide sequence and characterization of erythromycin resistance determinant that encodes macrolide 2'-phosphotransferase I in Escherichia coli.

作者信息

Noguchi N, Emura A, Matsuyama H, O'Hara K, Sasatsu M, Kono M

机构信息

Department of Microbiology, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Japan.

出版信息

Antimicrob Agents Chemother. 1995 Oct;39(10):2359-63. doi: 10.1128/AAC.39.10.2359.

DOI:10.1128/AAC.39.10.2359
PMID:8619599
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC162946/
Abstract

The DNA fragment (3.3 kb) containing the erythromycin resistance determinant was cloned from Escherichia coli Tf481A and sequenced. Deletion and complementation analyses indicated that the expression of high-level resistance to erythromycin requires two genes, mphA and mrx, which encode macrolide 2'-phosphotransferase I and an unidentified hydrophobic protein, respectively.

摘要

从大肠杆菌Tf481A中克隆出含有红霉素抗性决定簇的DNA片段（3.3 kb）并进行测序。缺失和互补分析表明，对红霉素的高水平抗性表达需要两个基因，即mphA和mrx，它们分别编码大环内酯2'-磷酸转移酶I和一种未知的疏水蛋白。

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Nucleotide sequence and characterization of erythromycin resistance determinant that encodes macrolide 2'-phosphotransferase I in Escherichia coli.编码大肠杆菌中红霉素2'-磷酸转移酶I的红霉素抗性决定子的核苷酸序列及特性
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本文引用的文献

1
Cloning of the DNA polymerase gene of Bacillus caldotenax and characterization of the gene product.嗜热栖热芽孢杆菌DNA聚合酶基因的克隆及基因产物的特性分析。
J Biochem. 1993 Mar;113(3):401-10. doi: 10.1093/oxfordjournals.jbchem.a124058.
2
A simple method for displaying the hydropathic character of a protein.一种展示蛋白质亲水性特征的简单方法。
J Mol Biol. 1982 May 5;157(1):105-32. doi: 10.1016/0022-2836(82)90515-0.
3
Tn1525, a kanamycin R determinant flanked by two direct copies of IS15.Tn1525，一种由两个IS15直接重复序列侧翼的卡那霉素抗性决定簇。
Mol Gen Genet. 1983;189(1):90-101. doi: 10.1007/BF00326060.
4
The kanamycin resistance transposon Tn2680 derived from the R plasmid Rts1 and carried by phage P1Km has flanking 0.8-kb-long direct repeats.源自R质粒Rts1并由噬菌体P1Km携带的卡那霉素抗性转座子Tn2680具有侧翼0.8 kb长的正向重复序列。
Plasmid. 1982 Sep;8(2):187-98. doi: 10.1016/0147-619x(82)90056-7.
5
Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.用核酸外切酶III进行单向消化可为DNA测序创建靶向断点。
Gene. 1984 Jun;28(3):351-9. doi: 10.1016/0378-1119(84)90153-7.
6
The origin of replication of plasmid p15A and comparative studies on the nucleotide sequences around the origin of related plasmids.质粒p15A的复制起点及相关质粒复制起点周围核苷酸序列的比较研究。
Cell. 1983 Jan;32(1):119-29. doi: 10.1016/0092-8674(83)90502-0.
7
Translational initiation in prokaryotes.原核生物中的翻译起始
Annu Rev Microbiol. 1981;35:365-403. doi: 10.1146/annurev.mi.35.100181.002053.
8
Properties of a R factor which originated in Pseudomonas aeruginosa 1822.源自铜绿假单胞菌1822的一种R因子的特性。
J Bacteriol. 1972 May;110(2):529-37. doi: 10.1128/jb.110.2.529-537.1972.
9
Rapid and efficient site-specific mutagenesis without phenotypic selection.无需表型选择的快速高效位点特异性诱变。
Methods Enzymol. 1987;154:367-82. doi: 10.1016/0076-6879(87)54085-x.
10
Structure of a phosphorylated derivative of oleandomycin, obtained by reaction of oleandomycin with an extract of an erythromycin-resistant strain of Escherichia coli.
J Antibiot (Tokyo). 1988 Jun;41(6):823-7. doi: 10.7164/antibiotics.41.823.