Haseley S R, Pantophlet R, Brade L, Holst O, Brade H
Division of Medical and Biochemical Microbiology, Research Centre Borstel, Centre for Medicine and Biosciences, Germany.
Eur J Biochem. 1997 Apr 15;245(2):477-81. doi: 10.1111/j.1432-1033.1997.t01-1-00477.x.
A polysaccharide containing rhamnose (Rha) and Gal was isolated by acetic acid hydrolysis, followed by gel-permeation chromatography, from the water-soluble lipopolysaccharide (phenol/water extracted) from Acinetobacter junii strain 65. The polysaccharide was characterised by means of monosaccharide analyses, Smith degradation, and NMR studies, and was shown to have a linear pentasaccharide repeating unit, as depicted below. This structure was specifically recognised in western blots and enzyme immunoassays by polyclonal rabbit antisera. [structure in text]
通过乙酸水解，随后进行凝胶渗透色谱法，从琼氏不动杆菌65株的水溶性脂多糖（酚/水提取）中分离出一种含有鼠李糖（Rha）和半乳糖（Gal）的多糖。通过单糖分析、史密斯降解和核磁共振研究对该多糖进行了表征，并显示其具有如下所示的线性五糖重复单元。在蛋白质免疫印迹和酶免疫测定中，这种结构被兔多克隆抗血清特异性识别。[文中结构]
