Bert F, Branger C, Lambert-Zechovsky N
Hôpital Beaujon, service de Microbiologie, 100 boulevard du Général Leclerc, 92210 Clichy, France.
Curr Microbiol. 1997 Apr;34(4):226-9. doi: 10.1007/s002849900173.
The SmaI restriction endonuclease digestion patterns of chromosomal DNAs from 99 pyogenic streptococci belonging to Lancefield group A (41 Streptococcus pyogenes), group C (seven S.dysgalactiae, 11 "S. equisimilis", three S. equi, eight S. zooepidemicus) and group G (25 human group G Streptococcus, four S. canis) were analyzed by pulsed-field gel electrophoresis (PFGE), and the results were compared with those previously obtained by multilocus enzyme electrophoresis (MLEE) and random amplified polymorphic DNA analysis (RAPD). PFGE revealed 93 distinct types among the 99 strains, and no patterns were common to strains of different species. The discriminatory power of PFGE was greater than that of MLEE and RAPD for groups A and G streptococci. The polymorphism among group C streptococci was similar with the three techniques. PFGE is, therefore, the most efficacious method for epidemiological typing of pyogenic streptococci.
通过脉冲场凝胶电泳（PFGE）分析了99株化脓性链球菌的染色体DNA的SmaI限制性核酸内切酶消化模式，这些化脓性链球菌属于A群（41株化脓性链球菌）、C群（7株乳房链球菌、11株“似马链球菌”、3株马链球菌、8株兽疫链球菌）和G群（25株人G群链球菌、4株犬链球菌），并将结果与先前通过多位点酶电泳（MLEE）和随机扩增多态性DNA分析（RAPD）获得的结果进行了比较。PFGE在99株菌株中显示出93种不同类型，不同物种的菌株没有共同的模式。对于A群和G群链球菌，PFGE的鉴别能力大于MLEE和RAPD。三种技术显示C群链球菌之间的多态性相似。因此，PFGE是化脓性链球菌流行病学分型的最有效方法。
