Biswas R, Ghosh P K, Sarkar M, Biswas N M
Department of Physiology, University of Calcutta, College of Science and Technology, India.
Life Sci. 1996;58(17):1469-74. doi: 10.1016/0024-3205(96)00117-8.
The purpose of the present study was to evaluate the effects of anti-alpha-2U-globulin on pituitary-gonadal functions in male rats. Adult male rats were given injections of anti-alpha-2u-globulin (1 mg/day) for 14 days, when they were killed 7 days after the last injection, serum levels of gonadotrophins and testosterone measured by radioimmunoassays, were less, testicular delta 3 beta- and 17 beta-hydroxy-steroid dehydrogenase (3 beta- and 17 beta-HSD) activities were suppressed, spermatogenesis was inhibited and serum level of alpha-2u-globulin was decreased in anti-alpha-2u-globulin treated rats. Administration of alpha-2u-globulin (1.5 mg/day) for 7 days to anti-alpha-2u-globulin treated rats reversed the 3beta-HSD and 17beta-HSD activities and serum levels of gonadotrophins, testosterone and alpha-2u-globulin, while spermatogenesis was restored to normal. The results indicate that changes in testicular steroidogenesis and spermatogenesis in rats after passive immunization against alpha-2u-globulin may be due to decrease in availability of endogenous alpha-2u-globulin.
本研究的目的是评估抗α-2U球蛋白对雄性大鼠垂体-性腺功能的影响。成年雄性大鼠注射抗α-2u球蛋白（1毫克/天），持续14天，在最后一次注射后7天处死，通过放射免疫分析法测定的促性腺激素和睾酮血清水平降低，抗α-2u球蛋白处理的大鼠睾丸δ3β-和17β-羟基类固醇脱氢酶（3β-和17β-HSD）活性受到抑制，精子发生受到抑制，血清α-2u球蛋白水平降低。给抗α-2u球蛋白处理的大鼠注射α-2u球蛋白（1.5毫克/天）7天，可逆转3β-HSD和17β-HSD活性以及促性腺激素、睾酮和α-2u球蛋白的血清水平，同时精子发生恢复正常。结果表明，被动免疫抗α-2u球蛋白后大鼠睾丸类固醇生成和精子发生的变化可能是由于内源性α-2u球蛋白可用性降低所致。
