Ma R Z, Russ I, Park C, Heyen D W, Beever J E, Green C A, Lewin H A
Department of Animal Sciences, University of Illinois at Urbana-Champaign 61801, USA.
Anim Genet. 1996 Feb;27(1):43-7. doi: 10.1111/j.1365-2052.1996.tb01175.x.
A small-insert bovine genomic library was constructed in pBluescript II SK(+) and enriched for microsatellites by selective rescue of single-stranded pBluescript DNA carrying (CA)n/(TG)n tandem repeats. Approximately 50% of the clones in the enriched library contained (CA)n repeats or CA-rich sequences. Sequencing of clones selected for (CA)n repeats resulted in the identification and characterization of 45 (CA)n polymorphic microsatellites. Genotyping in 9 large paternal half-sib families indicated that 40 of these microsatellite markers exhibit autosomal Mendelian inheritance. The numbers of alleles range from 2 to 18, with an average of 6.3 per locus. The polymorphic microsatellite markers we have identified and characterized will contribute to the construction of a high-resolution linkage map of bovine genome.
构建了一个插入片段较小的牛基因组文库，该文库采用pBluescript II SK(+)载体，并通过选择性拯救携带(CA)n/(TG)n串联重复序列的单链pBluescript DNA来富集微卫星。富集文库中约50%的克隆含有(CA)n重复序列或富含CA的序列。对选择的含有(CA)n重复序列的克隆进行测序，鉴定并表征了45个(CA)n多态微卫星。在9个大型父系半同胞家系中进行基因分型表明，这些微卫星标记中有40个表现出常染色体孟德尔遗传。等位基因数量范围为2至18个，平均每个位点6.3个。我们鉴定和表征的多态微卫星标记将有助于构建牛基因组的高分辨率连锁图谱。
