Cercenado E, Unal S, Eliopoulos C T, Rubin L G, Isenberg H D, Moellering R C, Eliopoulos G M
Deaconess Hospital, Boston, Massachusetts 02215, USA.
J Antimicrob Chemother. 1995 Nov;36(5):821-5. doi: 10.1093/jac/36.5.821.
During investigation of an outbreak of vancomycin resistant Enterococcus faecium in a paediatric hospital, an isolate of Enterococcus durans resistant to vancomycin, teicoplanin, ampicillin and highly resistant to gentamicin and streptomycin was found in the stools of a patient also colonized with a strain of E. faecium with the same resistance pattern. Minimal inhibitory concentrations of vancomycin and teicoplanin were 512 and 64 mg/mL, respectively. Resistance to vancomycin as well as high-level resistance to gentamicin was transferable to an E. faecium recipient strain. Both multiresistant E. faecium and E. durans isolates as well as the transconjugant presented only one plasmid. The vanA gene was detected and localized to the high molecular weight plasmid by DNA hybridization with a vanA gene probe. Growth in vancomycin resulted in induction of an approximately 40 kDa protein visible in membrane preparations from these cells. Genetic linkage between vancomycin and gentamicin resistance genes in the same plasmid is suggested.
在一家儿科医院对耐万古霉素屎肠球菌暴发进行调查期间，在一名同时定植有具有相同耐药模式的屎肠球菌菌株的患者粪便中，发现了一株对万古霉素、替考拉宁、氨苄西林耐药且对庆大霉素和链霉素高度耐药的耐久肠球菌分离株。万古霉素和替考拉宁的最低抑菌浓度分别为512和64mg/mL。对万古霉素的耐药性以及对庆大霉素的高水平耐药性可转移至屎肠球菌受体菌株。多重耐药的屎肠球菌和耐久肠球菌分离株以及转接合子均仅呈现一个质粒。通过与vanA基因探针进行DNA杂交，检测到vanA基因并将其定位到高分子量质粒上。在万古霉素中生长导致在这些细胞的膜制剂中可见一种约40kDa的蛋白质被诱导产生。提示在同一质粒中万古霉素和庆大霉素耐药基因之间存在遗传连锁。
