Merayo-Lloves J, Zhao T Z, Dutt J E, Foster C S
Hilles Immunology Laboratory, Massachusetts Eye & Ear Infirmary, Boston, USA.
J Allergy Clin Immunol. 1996 May;97(5):1129-40. doi: 10.1016/s0091-6749(96)70268-3.
Allergic conjunctivitis is the most common atopic disease affecting the eye. To study the pathophysiology and effectiveness of antiallergic drugs, it is necessary to develop animal models that closely mimic human allergic conjunctivitis.
The study was performed to develop an experimental murine model of ocular allergic conjunctivitis to an airborne allergen.
SWR/J mice were divided into the following groups: group 1, untreated, experimental; group 2, phosphate-buffered saline-treated; group 3, nedocromil sodium-treated; and group 4, unmanipulated controls. Groups 1, 2, and 3 were exposed to ragweed by topical contact with the nasal and conjunctival mucosae. Allergic conjunctivitis was evaluated by scoring of clinical signs, serum IgE levels, and histologic findings.
Mice exposed to ragweed had clinicopathologic signs of allergic conjunctivitis and specific anti-ragweed IgE. Allergic conjunctivitis was modulated by nedocromil sodium. Treated mice had fewer clinical signs of allergy, lower levels of ragweed-specific IgE, reduction of conjunctival eosinophil infiltration, decrease in the number of intact and degranulating mast cells, and reduction of cytokine release.
This is the first report of a murine model of allergic conjunctivitis to an airborne allergen that can be used to study the disease pathophysiology and its response to treatment.
过敏性结膜炎是影响眼部最常见的特应性疾病。为研究抗过敏药物的病理生理学及疗效，有必要建立能紧密模拟人类过敏性结膜炎的动物模型。
本研究旨在建立一种针对空气传播变应原的实验性小鼠眼部过敏性结膜炎模型。
将SWR/J小鼠分为以下几组：第1组，未处理的实验组；第2组，磷酸盐缓冲液处理组；第3组，奈多罗米钠处理组；第4组，未处理的对照组。第1、2和3组通过局部接触鼻腔和结膜黏膜暴露于豚草。通过对临床体征、血清IgE水平和组织学结果进行评分来评估过敏性结膜炎。
暴露于豚草的小鼠出现过敏性结膜炎的临床病理体征及特异性抗豚草IgE。奈多罗米钠可调节过敏性结膜炎。经处理的小鼠过敏临床体征较少，豚草特异性IgE水平较低，结膜嗜酸性粒细胞浸润减少，完整和脱颗粒肥大细胞数量减少，细胞因子释放减少。
这是关于针对空气传播变应原的小鼠过敏性结膜炎模型的首次报道，该模型可用于研究疾病病理生理学及其对治疗的反应。
