Garcia-Valcarcel M, Doel T, Collen T, Ryan M, Parkhouse R M
British Biotech Pharmaceutical Ltd., Oxford, UK.
J Gen Virol. 1996 Apr;77 ( Pt 4):727-35. doi: 10.1099/0022-1317-77-4-727.
The role of T cells in immunity to foot-and-mouth disease virus is still poorly defined compared to that of the humoral response. In this paper we describe a systematic, longitudinal study on the cellular recognition of FMDV and its subunit protein VP1 by bovine peripheral blood T lymphocytes. Multiple vaccination with a single virus serotype induced a serotype cross-reactive proliferative T cell repertoire that varied in magnitude between individual animals and with the serotype of the vaccine used. Primary proliferative T cell responses of vaccinated and acutely infected cattle were weak relative to the magnitude of responses determined for the same animals after boosting. In contrast, the level of circulating antibody produced after both primary and secondary exposure to virus was good. Phenotypic analysis of lymphocytes from vaccinated or infected cattle showed a small increase in CD8+ T cells after infection compared to vaccination. However, in general the profiles of circulating lymphocytes elicited were similar. Thus, we were not able to use proliferative or phenotypic analyses to distinguish between vaccinated and convalescent cattle. T cell recognition of VP1 by multiply-vaccinated cattle was serotype-specific implying that the cross-reactive responses observed with whole virus may be attributed to proteins other than VP1. In contrast to other studies, immunization with recombinant VP1 induced only low levels of neutralizing antibody and failed to elicit profound proliferative responses or protection ever after two immunizations.
与体液免疫反应相比，T细胞在口蹄疫病毒免疫中的作用仍未明确界定。在本文中，我们描述了一项关于牛外周血T淋巴细胞对口蹄疫病毒及其亚基蛋白VP1的细胞识别的系统性纵向研究。用单一病毒血清型多次接种诱导了一种血清型交叉反应性增殖性T细胞库，其大小在个体动物之间以及与所用疫苗的血清型之间有所不同。相对于加强免疫后对同一动物测定的反应强度，接种疫苗和急性感染牛的原发性增殖性T细胞反应较弱。相比之下，初次和二次接触病毒后产生的循环抗体水平良好。对接种或感染牛的淋巴细胞进行表型分析表明，与接种疫苗相比，感染后CD8 + T细胞略有增加。然而，总体而言，所引发的循环淋巴细胞谱相似。因此，我们无法通过增殖或表型分析来区分接种疫苗的牛和康复牛。多次接种牛对VP1的T细胞识别具有血清型特异性，这意味着用全病毒观察到的交叉反应性反应可能归因于VP1以外的蛋白质。与其他研究不同，用重组VP1免疫仅诱导低水平的中和抗体，并且即使在两次免疫后也未能引发强烈的增殖反应或保护作用。
