Stewart A C, Eriksson A M, Manos M M, Muñoz N, Bosch F X, Peto J, Wheeler C M
Department of Cell Biology and the New Mexico Cancer Research and Treatment Center, University of New Mexico School of Medicine, Albuquerque 87131, USA.
J Virol. 1996 May;70(5):3127-36. doi: 10.1128/JVI.70.5.3127-3136.1996.
In this study, we have examined intratype human papillomavirus (HPV) sequence variation in a worldwide collection of cervical specimens. Twelve different HPV types including HPV-18, HPV-33, HPV-35, HPV-39, HPV-45, HPV-51, HPV-52, HPV-58, HPV-59, HPV-68 (ME180), MM9/PAP238A (recently designated HPV-73), and a novel partial genomic HPV sequence designated MM4/Wl3B were analyzed in this study. Cervical specimens were collected as part of epidemiological investigations conducted in New Mexico and an international study of invasive cervical cancer (IBSCC). Specimens from several countries including Argentina, Brazil, Bolivia, Benin, Cuba, Colombia, Chile, Germany, Mali, Panama, Paraguay, Spain, Algeria, Uganda, Guinea, Tanzania, Indonesia, Philippines, Thailand, and the United States were evaluated. Specimen DNAs were subjected to amplification with the MY09/11 L1 consensus PCR system. The PCR products were cloned, and an approximately 410-bp region in the L1 open reading frame was sequenced from 146 specimens (approximately 60,000 bp). Within a single HPV type, nucleotide diversity varied between 0.2 and 2.9% (i.e., between any pair of variants) and the majority of nucleotide changes were synonymous (amino acid conserving). These data provide information pertinent to HPV diagnostic probe development and are potentially relevant to future rational vaccine strategies. Similarly, amino acid diversity varied between 0 and 5.1%. Some of these amino acid changes may represent markers of intertype evolutionary relationships. Presuming that HPVs have evolved under the same constraints as their corresponding hosts, the limited genetic diversity observed for all HPVs studied to date may reflect an evolutionary bottleneck occurring in both virus and host populations.
在本研究中，我们检测了来自世界各地的宫颈标本中人类乳头瘤病毒（HPV）的型内序列变异。本研究分析了12种不同的HPV类型，包括HPV-18、HPV-33、HPV-35、HPV-39、HPV-45、HPV-51、HPV-52、HPV-58、HPV-59、HPV-68（ME180）、MM9/PAP238A（最近命名为HPV-73）以及一个新的部分基因组HPV序列MM4/Wl3B。宫颈标本是在新墨西哥州进行的流行病学调查以及一项国际侵袭性宫颈癌研究（IBSCC）中收集的。对来自多个国家的标本进行了评估，这些国家包括阿根廷、巴西、玻利维亚、贝宁、古巴、哥伦比亚、智利、德国、马里、巴拿马、巴拉圭、西班牙、阿尔及利亚、乌干达、几内亚、坦桑尼亚、印度尼西亚、菲律宾、泰国和美国。标本DNA用MY09/11 L1一致性PCR系统进行扩增。PCR产物被克隆，并且从146个标本（约60,000 bp）中对L1开放阅读框中一个约410 bp的区域进行了测序。在单一HPV类型内，核苷酸多样性在0.2%至2.9%之间变化（即任意两个变体之间），并且大多数核苷酸变化是同义的（氨基酸保守）。这些数据为HPV诊断探针的开发提供了相关信息，并且可能与未来合理的疫苗策略相关。同样，氨基酸多样性在0至5.1%之间变化。其中一些氨基酸变化可能代表型间进化关系的标记。假设HPV与其相应宿主在相同的限制条件下进化，那么迄今为止所研究的所有HPV中观察到的有限遗传多样性可能反映了病毒和宿主群体中发生的进化瓶颈。
