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去污剂曲拉通X-100对嗜热真菌硫磺马尔布兰奇霉菌生长及α-葡萄糖苷酶产生的影响。

Effect of a detergent Triton X-100 on growth and alpha-glucosidase production by the thermophilic fungus Malbranchea sulfurea.

作者信息

Gupta A K, Gautam S P

机构信息

Department of Biological Science, Rani Durgavati University, Jabalpur, India.

出版信息

Microbios. 1995;84(339):127-30.

PMID:8628122
Abstract

A detergent Triton X-100 was found to affect maltose-induced synthesis of extracellular, mycelial and intracellular alpha-glucosidase in the thermophilic fungus Malbranchea sulfurea. The extracellular fraction of the total alpha-glucosidase yield was found to be 90.7% and 40.4% in the presence and absence of the detergent, respectively. Data suggest that supplementation of the detergent in the medium resulted in the partial solubilization of the cell-bound alpha-glucosidase and caused its release in the growth medium.

摘要

已发现去污剂曲拉通X-100会影响嗜热真菌硫黄马尔布兰奇霉菌中麦芽糖诱导的胞外、菌丝体和胞内α-葡萄糖苷酶的合成。在有和没有该去污剂的情况下,总α-葡萄糖苷酶产量的胞外部分分别为90.7%和40.4%。数据表明,在培养基中添加去污剂会导致细胞结合的α-葡萄糖苷酶部分溶解,并使其释放到生长培养基中。

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