Dick T, Surana U, Chia W
Drosphila Neurobiology Laboratory, National University of Singapore, Republic of Singapore.
Mol Gen Genet. 1996 Apr 24;251(1):38-43. doi: 10.1007/BF02174342.
Cytoplasmic dynein is a multisubunit, microtubule-dependent motor enzyme that has been proposed to function in a variety of intracellular movements. As part of an effort to understand the evolution and the biological roles of cytoplasmic dynein, we have identified the first non-metazoan dynein light chain 1, SLC1, in the yeast Saccharomyces cerevisiae. The amino acid sequence of the SLC1 protein is similar to those of the human, Drosophila and Caenorhabditis cytoplasmic dynein light chains 1. The SLC1 gene lies adjacent to the YAP2 (= CAD1) transcription unit. The SLC1 coding sequence is split by two introns and its mRNA is detectable throughout the cell cycle. Tetrad analysis of heterozygotes harboring a TRP insertion in the SLC1 coding region indicate that SLC1 function is not essential for cell viability. Furthermore, we demonstrate that double mutants, defective for SLC1 and the kinesin-related CIN8 genes are non-lethal. The redundancy of SLC1 function in yeast contrasts with the cell death caused by loss-of-function mutations in the dynein light chain 1 gene in Drosophila melanogaster.
胞质动力蛋白是一种多亚基的、依赖微管的运动酶，有人提出它在多种细胞内运动中发挥作用。作为了解胞质动力蛋白的进化和生物学作用的一部分工作，我们在酿酒酵母中鉴定出了首个非后生动物的动力蛋白轻链1，即SLC1。SLC1蛋白的氨基酸序列与人类、果蝇和秀丽隐杆线虫的胞质动力蛋白轻链1的序列相似。SLC1基因位于YAP2（= CAD1）转录单元附近。SLC1编码序列被两个内含子分隔，其mRNA在整个细胞周期中都可检测到。对在SLC1编码区插入TRP的杂合子进行四分体分析表明，SLC1功能对细胞活力并非必不可少。此外，我们证明，SLC1和与驱动蛋白相关的CIN8基因均有缺陷的双突变体并非致命。酵母中SLC1功能的冗余与黑腹果蝇中动力蛋白轻链1基因功能丧失突变导致的细胞死亡形成对比。
