Minissi S, Ciccotti E, Rizzoni M
Dipartimento di Biologia, Università degli Studi di Roma Tor Vergata, via della Ricerca Scientifica, Rome, Italy.
Mutat Res. 1996 Apr 6;367(4):245-51. doi: 10.1016/s0165-1218(96)90084-1.
Erythrocyte micronucleus frequencies in wild fish from two riverine environments and in fish reproduced and reared under controlled conditions (control group) were compared, with the aim to evaluate the suitability of the MN test for the in situ detection of mutagens in freshwaters. Fish were caught in different months in two rivers of central Italy which have different pollution levels. As indicator species, the barbel (Barbus plebejus) was chosen because of its ecological significance. Blood samplings were performed on wild fish immediately after capture and repeated at different time intervals on the same individuals, which were maintained in controlled conditions after capture. A total of 10,000 erythrocytes per specimen were scored. No significant differences in micronucleus frequencies were observed between the control group and fish from the unpolluted river (Mignone). A significantly higher frequency of micronuclei was observed in fish caught in the polluted river (Tiber), in comparison to both the controls and the Mignone river fish. No significant seasonal differences were observed. Barbels examined 50 and 100 days after capture presented a remarkable decrease in micronucleus frequency in comparison with the frequency observed in barbels at capture. The micronucleus test in fish erythrocytes was shown to be a sensitive bioassay for detecting mutagenic pollution in fresh water environments.
比较了来自两种河流环境的野生鱼类以及在受控条件下繁殖和饲养的鱼类（对照组）的红细胞微核频率，目的是评估微核试验用于原位检测淡水中诱变剂的适用性。在意大利中部两条污染程度不同的河流中，于不同月份捕获鱼类。由于其生态意义，选择了鲤科鱼（Barbus plebejus）作为指示物种。对捕获后的野生鱼类立即进行血样采集，并在捕获后将同一条鱼在受控条件下饲养，在不同时间间隔重复采样。每个样本共计数10,000个红细胞。对照组与未受污染河流（米尼奥内河）中的鱼类之间，未观察到微核频率有显著差异。与对照组和米尼奥内河的鱼类相比，在受污染河流（台伯河）中捕获的鱼类微核频率显著更高。未观察到显著的季节差异。捕获后50天和100天检查的鲤科鱼，与捕获时观察到的微核频率相比，微核频率显著降低。鱼类红细胞微核试验被证明是检测淡水环境中诱变污染的灵敏生物测定方法。
