Niemhom S, Maeda S, Raksakait K, Petchclai B
Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
Southeast Asian J Trop Med Public Health. 1995;26 Suppl 1:325-8.
Nasopharyngeal carcinoma (NPC) is one of the most common malignancies in Thailand. The association of Epstein-Barr virus (EBV) and NPC, especially undifferentiated type, has been documented. There is, however, conflicting evidence with regard to the squamous cell type. We have therefore investigated EBV-DNA in all the three types of NPC to assess the association of EBV and NPC in Thai patients. EBV-DNA was detected in formalin-fixed paraffin-embedded tissues from the patients of Ramathibodi Hospital, Bangkok using polymerase chain reaction. A primer pair that amplified EBV nuclear antigen gene was used in the reactions and the amplification products were hybridized with a specific EBV probe. EBV-DNA was identified in 24 of 28 of tissue samples from patients with undifferentiated NPC, 25 of 40 samples from patients with squamous cell NPC and 3 of 4 samples with nonkeratinizing NPC. None of 12 nasopharyngeal tissue samples without NPC contained detectable EBV-DNA. Our results indicated a strong association of EBV with undifferentiated as well as non-keratinizing NPC. EBV-DNA was demonstrated in most cases of squamous cell NPC but the association of EBV in this type of carcinoma was not as frequent as in the other two types of NPC.
鼻咽癌（NPC）是泰国最常见的恶性肿瘤之一。爱泼斯坦-巴尔病毒（EBV）与NPC，尤其是未分化型NPC之间的关联已有文献记载。然而，关于鳞状细胞型NPC，证据存在矛盾。因此，我们对所有三种类型的NPC进行了EBV-DNA检测，以评估泰国患者中EBV与NPC的关联。使用聚合酶链反应在曼谷拉玛提波迪医院患者的福尔马林固定石蜡包埋组织中检测EBV-DNA。反应中使用一对扩增EBV核抗原基因的引物，并将扩增产物与特异性EBV探针杂交。在28例未分化NPC患者的组织样本中有24例检测到EBV-DNA，40例鳞状细胞NPC患者样本中有25例，4例非角化NPC患者样本中有3例。12例无NPC的鼻咽组织样本中均未检测到可检测到的EBV-DNA。我们的结果表明EBV与未分化型以及非角化型NPC密切相关。大多数鳞状细胞NPC病例中都检测到了EBV-DNA，但EBV在这种类型的癌症中的关联不如在其他两种类型的NPC中频繁。
