Beilke M A, Traina-Dorge V, England J D, Blanchard J L
Tulane University Medical School, New Orleans, Louisiana 70112, USA.
Arthritis Rheum. 1996 Apr;39(4):610-5. doi: 10.1002/art.1780390410.
To establish a simian model of human T lymphotropic virus type I (HTLV-I) infection and disease.
Irradiated HTLV-I-producing cells were used to infect two 2-year-old rhesus macaque monkeys (Macaca mulatta). One monkey was also simultaneously inoculated with a cell-free suspension of simian immunodeficiency virus (SIV). Evidence of infection was monitored by serial clinical examinations and by serologic, molecular, and virologic assays.
Both HTLV-I-inoculated monkeys became persistently infected following inoculation. Clinical disease was observed in the singly inoculated monkey, which developed arthritis (with synovial fluid positive for HTLV-I by culture and polymerase chain reaction), anterior chamber uveitis, and steroid-responsive polymyositis confirmed by electrophysiologic studies. The dually inoculated animal remained clinically healthy, despite high levels of SIV and HTLV-I virus expression and loss of HTLV-I-specific antibodies.
These results indicate the utility of a nonhuman primate model for studying HTLV-I disease pathogenesis and the dynamics of SIV-1/HTLV-I retroviral coinfection.
建立I型人类嗜T淋巴细胞病毒（HTLV-I）感染及相关疾病的猕猴模型。
用经辐射的产生HTLV-I的细胞感染两只2岁的恒河猴（猕猴）。其中一只猴子还同时接种了猿猴免疫缺陷病毒（SIV）的无细胞悬液。通过系列临床检查以及血清学、分子生物学和病毒学检测来监测感染证据。
两只接种HTLV-I的猴子在接种后均持续感染。在单接种的猴子中观察到临床疾病，其出现了关节炎（滑膜液经培养和聚合酶链反应检测HTLV-I呈阳性）、前房葡萄膜炎以及经电生理研究证实的类固醇反应性多发性肌炎。双接种的动物尽管SIV和HTLV-I病毒表达水平很高且HTLV-I特异性抗体消失，但仍保持临床健康。
这些结果表明非人类灵长类动物模型在研究HTLV-I疾病发病机制以及SIV-1/HTLV-I逆转录病毒合并感染动态方面具有实用性。
