秀丽隐杆线虫Gqα基因的突变会破坏运动、产卵和生存能力。
Mutations in a C. elegans Gqalpha gene disrupt movement, egg laying, and viability.
作者信息
Brundage L, Avery L, Katz A, Kim U J, Mendel J E, Sternberg P W, Simon M I
机构信息
Division of Biology and Howard Hughes Medical Institute of Technology, Pasadena, California 91125, USA.
出版信息
Neuron. 1996 May;16(5):999-1009. doi: 10.1016/s0896-6273(00)80123-3.
Abstract
We find that C. elegans egl-30 encodes a heterotrimeric G protein a subunit more than 80% identical to mammalian Gqalpha family proteins, and which can function as a Gqalpha subunit in COS-7 cells. We have identified new egl-30 alleles in a selection for genes involved in the C. elegans acetylcholine response. Two egl-30 alleles specify premature termination of Gqalpha and are essentially lethal in homozygotes. Animals homozygous for six other egl-30 alleles are viable and fertile, but exhibit delayed egg laying and leave flattened tracks. Overexpression of the wild-type egl-30 gene produces the opposite behavior. Analysis of these mutants suggest that their phenotypes reflect defects in the muscle or neuromuscular junction.
摘要
我们发现，秀丽隐杆线虫的egl-30编码一种异源三聚体G蛋白α亚基，与哺乳动物Gqα家族蛋白的同源性超过80%，并且在COS-7细胞中可作为Gqα亚基发挥作用。我们在筛选参与秀丽隐杆线虫乙酰胆碱反应的基因时鉴定出了新的egl-30等位基因。两个egl-30等位基因导致Gqα提前终止，纯合子基本致死。其他六个egl-30等位基因的纯合动物可存活且可育，但产卵延迟且留下扁平的轨迹。野生型egl-30基因的过表达则产生相反的行为。对这些突变体的分析表明，它们的表型反映了肌肉或神经肌肉接头的缺陷。
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