Shi Y F, Xie X, Zhao C L, Ye D F, Lu S M, Hor J J, Pao C C
Women's Hospital, Zhejiang Medical University, Hangzhou, China.
Br J Cancer. 1996 May;73(10):1216-9. doi: 10.1038/bjc.1996.233.
The objectives of this study were to better our understanding of the carcinogenesis of gestational trophoblastic tumours and to investigate the possible presence of mutational alteration of the p53 tumour-suppressor gene in these tumours. Amplification-based direct DNA sequencing was performed on 14 hydatidiform moles, six invasive moles, eight choriocarcinomas and ten normal early placental tissues. No mutation in exons 5-8 was detected in any of these 38 tissue specimens. These results suggest that a mutation in p53 tumour suppressor either does not exist or is a very rare event in gestational trophoblastic tumours. The gestational trophoblastic tumours probably involve a tumour-suppressor gene other than p53 gene or may follow a completely different pathway to their malignant phenotype.
本研究的目的是加深我们对妊娠滋养细胞肿瘤致癌作用的理解，并调查这些肿瘤中p53肿瘤抑制基因可能存在的突变改变。对14例葡萄胎、6例侵蚀性葡萄胎、8例绒毛膜癌和10例正常早期胎盘组织进行了基于扩增的直接DNA测序。在这38个组织标本中，未检测到外显子5-8的突变。这些结果表明，p53肿瘤抑制基因的突变在妊娠滋养细胞肿瘤中要么不存在，要么是非常罕见的事件。妊娠滋养细胞肿瘤可能涉及p53基因以外的肿瘤抑制基因，或者可能遵循完全不同的途径形成其恶性表型。
