免疫细胞化学和聚合酶链反应技术检测乳腺癌中雌激素受体和转化生长因子β的比较
Comparison between immunocytochemical and polymerase chain reaction techniques for detection of oestrogen receptor and transforming growth factor beta in breast cancer.
作者信息
Amoils K D, Seymour L, Bezwoda W R
机构信息
Department of Medicine, University of Witwatersrand, Parktown, South Africa.
出版信息
Br J Cancer. 1996 May;73(10):1255-9. doi: 10.1038/bjc.1996.240.
The utility of the polymerase chain reaction (PCR) as a technique for determining the expression of transforming growth factor beta (TGF-beta) and of the oestrogen receptor (ER) in clinical breast cancer tissue was examined. PCR analysis was compared with immunocytochemical assays for TGF-beta and for ER. Seventy confirmed breast carcinoma samples were analysed for ER using both techniques with a statistically highly significant concordance (P < 0.001) between the two methods. Nineteen samples were observed to be ER positive and 46 samples were found to be ER negative by both techniques. Forty-eight samples were analysed for TGF-beta using both PCR and immunocytochemistry. Of the 24 samples observed to be positive for TGF-beta by immunocytochemistry, all were found to be positive for TGF-beta mRNA (PCR). Similarly, the 24 samples observed to be TGF-beta negative by immunocytochemistry were also negative for TGF-beta mRNA, indicating 100% specificity and 100% sensitivity of the PCR technique. PCR is therefore considered a viable technique for analysis of both ER and TGF-beta in small samples such as fine-needle aspirates.
研究了聚合酶链反应（PCR）作为一种检测临床乳腺癌组织中转化生长因子β（TGF-β）和雌激素受体（ER）表达的技术的实用性。将PCR分析与TGF-β和ER的免疫细胞化学检测进行了比较。使用这两种技术对70份确诊的乳腺癌样本进行了ER分析，两种方法之间具有统计学上高度显著的一致性（P < 0.001）。两种技术均观察到19个样本为ER阳性，46个样本为ER阴性。使用PCR和免疫细胞化学对48个样本进行了TGF-β分析。在免疫细胞化学检测中观察到的24个TGF-β阳性样本中，所有样本的TGF-β mRNA（PCR）均为阳性。同样，在免疫细胞化学检测中观察到的24个TGF-β阴性样本的TGF-β mRNA也为阴性，表明PCR技术具有100%的特异性和100%的敏感性。因此，PCR被认为是一种可行的技术，可用于分析细针穿刺等小样本中的ER和TGF-β。
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