Clouthier D E, Avarbock M R, Maika S D, Hammer R E, Brinster R L
Laboratory of Reproductive Physiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia 19104, USA.
Nature. 1996 May 30;381(6581):418-21. doi: 10.1038/381418a0.
Recently, transplantation of mouse donor spermatogonial stem cells from a fertile testis to an infertile recipient mouse testis was described. The donor cells established spermatogenesis in the seminiferous tubules of the host, and normal spermatozoa were produced. In the most successful transplants, the recipient mice were fertile and sired up to 80 per cent of progeny from donor cells. Here we examine the feasibility of transplanting spermatogonial stem cells from other species to the mouse seminiferous tubule to generate spermatogenesis. Marked testis cells from transgenic rats were transplanted to the testes of immunodeficient mice, and in all of 10 recipient mice (in 19 of 20 testes), rat spermatogenesis occurred. Epididymides of eight mice were examined, and the three from mice with the longest transplants (> or = 110 days) contained rat spermatozoa with normal morphology. The generation of rat spermatogenesis in mouse testes suggests that spermatogonial stem cells of many species could be transplanted, and opens the possibility of xenogeneic spermatogenesis for other species.
最近，有人描述了将来自可育睾丸的小鼠供体精原干细胞移植到不育受体小鼠睾丸的过程。供体细胞在宿主的生精小管中建立了精子发生过程，并产生了正常的精子。在最成功的移植中，受体小鼠具有生育能力，其后代中高达80%来自供体细胞。在此，我们研究了将其他物种的精原干细胞移植到小鼠生精小管以产生精子发生的可行性。将来自转基因大鼠的标记睾丸细胞移植到免疫缺陷小鼠的睾丸中，在所有10只受体小鼠中（20个睾丸中的19个），均发生了大鼠精子发生。检查了8只小鼠的附睾，来自移植时间最长（≥110天）的小鼠的3个附睾中含有形态正常的大鼠精子。在小鼠睾丸中产生大鼠精子发生表明，许多物种的精原干细胞都可以进行移植，并为其他物种的异种精子发生开辟了可能性。
