Sabie F T, Gadd G M
Department of Biological Sciences, University of Dundee, Scotland, UK.
Mycopathologia. 1992 Sep;119(3):147-56. doi: 10.1007/BF00448812.
A yeast-mycelium (Y-M) transition in Candida albicans was induced by exogenous yeast extract, adenosine, adenosine 5'-monophosphate (AMP), adenosine 5'-diphosphate (ADP), adenosine 3':5' cyclic monophosphate (cAMP) and its analogue N6, O2'-dibutyryl adenosine 3':5'-cyclic monophosphate (dbcAMP) in defined liquid medium at 25 degrees C. Adenosine 5'-triphosphate (ATP) was found to delay germ tube formation in yeast cells, whereas the cAMP phosphodiesterase inhibitors, theophylline and caffeine, induced a Y-M transition. Intracellular and extracellular cyclic AMP levels increased during the yeast-mycelium transition and maximum levels of intracellular cyclic AMP coincided with maximum germ tube formation. Of the many inducers and inhibitors of germ tube and mycelium formation in C. albicans tested, including incubation at 37 degrees C or in the presence of 1.5 mM CaCl2, the calmodulin inhibitor calmidazolium (R24571) added together with CaCl2 induced the highest intra- and extracellular cyclic AMP levels. These results confirm the involvement of cyclic AMP in the yeast-mycelium transition of C. albicans.
在25摄氏度的特定液体培养基中，外源酵母提取物、腺苷、5'-单磷酸腺苷（AMP）、5'-二磷酸腺苷（ADP）、3':5'-环磷酸腺苷（cAMP）及其类似物N6, O2'-二丁酰基-3':5'-环磷酸腺苷（dbcAMP）可诱导白色念珠菌发生酵母-菌丝体（Y-M）转变。发现5'-三磷酸腺苷（ATP）可延迟酵母细胞中芽管的形成，而cAMP磷酸二酯酶抑制剂茶碱和咖啡因可诱导Y-M转变。在酵母-菌丝体转变过程中，细胞内和细胞外的环磷酸腺苷水平升高，细胞内环磷酸腺苷的最高水平与芽管形成的最大值一致。在测试的众多白色念珠菌芽管和菌丝体形成的诱导剂和抑制剂中，包括在37摄氏度或1.5 mM氯化钙存在下孵育，与氯化钙一起添加的钙调蛋白抑制剂卡米达唑（R24571）可诱导最高的细胞内和细胞外环磷酸腺苷水平。这些结果证实了环磷酸腺苷参与了白色念珠菌的酵母-菌丝体转变。
