Munin Egberto, Giroldo Ligia Maria, Alves Leandro Procópio, Costa Maricilia Silva
Universidade do Vale do Paraíba - UNIVAP, Instituto de Pesquisa e Desenvolvimento - IP&D, Av. Shishima Hifumi, 2911, CEP12244-000 São José dos Campos, SP, Brazil.
J Photochem Photobiol B. 2007 Jul 27;88(1):16-20. doi: 10.1016/j.jphotobiol.2007.04.011. Epub 2007 May 8.
Due to the augmented number of immunocompromised patients, the infections associated to the pathogen of the genus Candida have increased dramatically in the recent years. In order to proliferate, Candida albicans can produce a germ tube formation extending from the cells. The germ tube formation is a transition state from budding to hyphal cells, and represents an essential stage for virulence. In this work we studied the effect of the photodynamic antimicrobial chemotherapy (PACT), a potential antimicrobial treatment on the germ tube formation by C. albicans. Germ tube formation was induced by goat serum after different treatments with Methylene blue (MB) and Laser (683nm). Our results demonstrated that photodynamic therapy using MB, as a photosensitizing drug; inhibits both the growth and the germ tube formation by C. albicans. Thus, our results suggest the possibility that Methylene blue, combined with light in a specific wavelength, can be used as a promising novel antifungal agent.
由于免疫功能低下患者数量的增加，近年来与念珠菌属病原体相关的感染急剧增加。为了增殖，白色念珠菌可产生从细胞延伸出的芽管形成。芽管形成是从芽生细胞到菌丝细胞的过渡状态，是毒力的一个重要阶段。在这项工作中，我们研究了光动力抗菌化疗（PACT），一种潜在的抗菌治疗方法，对白色念珠菌芽管形成的影响。在用亚甲蓝（MB）和激光（683nm）进行不同处理后，用山羊血清诱导芽管形成。我们的结果表明，使用MB作为光敏药物的光动力疗法可抑制白色念珠菌的生长和芽管形成。因此，我们的结果表明，亚甲蓝与特定波长的光结合，有可能作为一种有前景的新型抗真菌剂。
