Figueredo A, Ibarra J L, Rodriguez A, Molino A M, Gomez-de la Concha E, Fernandez-Cruz A, Patiño R
Center for the Study of Hypertension, Diabetes and Obesity, Hospital Universitario de San Carlos, Madrid, Spain.
Clin Immunol Immunopathol. 1996 Jun;79(3):252-5. doi: 10.1006/clin.1996.0076.
We measured class-specific antibodies to the mycobacterial hsp70 protein in 67 patients with diabetes mellitus (27 type 1 and 40 type 2) with or without vascular complications. Using ELISA, the levels of IgG and IgM antibodies in the sera of diabetic patients did not significantly differ either from those of healthy control subjects or between both types of diabetes, regardless of gender, disease duration, HbA1 level, or type of vascular complication. In patients with type 2 diabetes, the mean serum IgA levels were significantly higher than those in their matched controls [274(71) mg/dl vs 208(88) mg/dl; P < 0.01]. In this group of patients, the IgA antibody titer was significantly correlated to the serum IgA level (r = 0.334; P < 0.01). Serological autoimmunity (IgG or IgM type) to hsp70 protein is common in both the normal and the diabetic population. The increased IgA levels and anti-hsp70 IgA titers in the sera of diabetics suggest a possible role of IgA in the pathogenesis of the vascular complications of diabetes mellitus.
我们检测了67例糖尿病患者（27例1型糖尿病和40例2型糖尿病）有无血管并发症时针对分枝杆菌hsp70蛋白的类别特异性抗体。采用酶联免疫吸附测定法（ELISA），糖尿病患者血清中的IgG和IgM抗体水平与健康对照者相比，以及在两种类型糖尿病之间，无论性别、病程、糖化血红蛋白（HbA1）水平或血管并发症类型如何，均无显著差异。在2型糖尿病患者中，血清IgA平均水平显著高于其匹配的对照组[274(71)mg/dl对208(88)mg/dl；P<0.01]。在这组患者中，IgA抗体滴度与血清IgA水平显著相关（r=0.334；P<0.01）。对hsp70蛋白的血清学自身免疫（IgG或IgM型）在正常人群和糖尿病患者中均很常见。糖尿病患者血清中IgA水平升高和抗hsp70 IgA滴度升高提示IgA在糖尿病血管并发症发病机制中可能起作用。
