鼠伤寒沙门氏菌LT2中PocR调节蛋白与钴胺素生物合成（cob）操纵子启动子区域相互作用的体外分析。
In vitro analysis of the interactions between the PocR regulatory protein and the promoter region of the cobalamin biosynthetic (cob) operon of Salmonella typhimurium LT2.
作者信息
Rondon M R, Escalante-Semerena J C
机构信息
Department of Bacteriology, University of Wisconsin-Madison 53706, USA.
出版信息
J Bacteriol. 1996 Apr;178(8):2196-203. doi: 10.1128/jb.178.8.2196-2203.1996.
Abstract
The PocR protein of Salmonella typhimurium LT2 was overexpressed and used to demonstrate in vitro that it specifically binds to the cobalamin biosynthetic operon (cob) promoter region. Evidence is presented to show that PocR DNA-binding activity in vitro is regulated by the effector molecule 1,2-propanediol. Deletion analysis of the cob promoter (Pcob) suggested that two regions upstream of the promoter are needed for optimal activation of Pcob by PocR in vivo. DNase I footprinting experiments demonstrated that PocR binds to two sites within Pcob. The transcription initiation site of cob mRNA in response to 1,2-propanediol was identified and shown to be different from the one reported for transcription initiation under anoxic conditions in the absence of 1,2-propanediol.
摘要
鼠伤寒沙门氏菌LT2的PocR蛋白被过量表达，并用于在体外证明它特异性结合钴胺素生物合成操纵子（cob）的启动子区域。有证据表明，体外PocR的DNA结合活性受效应分子1,2-丙二醇调节。对cob启动子（Pcob）的缺失分析表明，启动子上游的两个区域是PocR在体内最佳激活Pcob所必需的。DNase I足迹实验证明PocR结合在Pcob内的两个位点。确定了响应1,2-丙二醇时cob mRNA的转录起始位点，并表明其与在无氧条件下且不存在1,2-丙二醇时报道的转录起始位点不同。
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1
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J Bacteriol. 1993 Jun;175(11):3317-26. doi: 10.1128/jb.175.11.3317-3326.1993.
2
Characterization of the cobalamin (vitamin B12) biosynthetic genes of Salmonella typhimurium.鼠伤寒沙门氏菌钴胺素（维生素B12）生物合成基因的特征分析
J Bacteriol. 1993 Jun;175(11):3303-16. doi: 10.1128/jb.175.11.3303-3316.1993.
3
Variation of half-site organization and DNA looping by AraC protein.AraC蛋白引起的半位点组织和DNA环化的变化。
EMBO J. 1993 Jan;12(1):35-44. doi: 10.1002/j.1460-2075.1993.tb05629.x.
4
Two global regulatory systems (Crp and Arc) control the cobalamin/propanediol regulon of Salmonella typhimurium.两种全局调控系统（Crp和Arc）控制鼠伤寒沙门氏菌的钴胺素/丙二醇调节子。
J Bacteriol. 1993 Nov;175(22):7200-8. doi: 10.1128/jb.175.22.7200-7208.1993.
5
The control region of the pdu/cob regulon in Salmonella typhimurium.鼠伤寒沙门氏菌中pdu/cob操纵子的调控区。
J Bacteriol. 1994 Sep;176(17):5474-82. doi: 10.1128/jb.176.17.5474-5482.1994.
6
The optimization of preparations of competent cells for transformation of E. coli.用于大肠杆菌转化的感受态细胞制备的优化。
Nucleic Acids Res. 1994 Jul 25;22(14):2857-8. doi: 10.1093/nar/22.14.2857.
7
DNA-dependent renaturation of an insoluble DNA binding protein. Identification of the RhaS binding site at rhaBAD.一种不溶性DNA结合蛋白的DNA依赖性复性。rhaBAD处RhaS结合位点的鉴定。
J Mol Biol. 1994 Nov 11;243(5):821-9. doi: 10.1006/jmbi.1994.1684.
8
The cobC gene of Salmonella typhimurium codes for a novel phosphatase involved in the assembly of the nucleotide loop of cobalamin.鼠伤寒沙门氏菌的cobC基因编码一种参与钴胺素核苷酸环组装的新型磷酸酶。
J Biol Chem. 1994 Oct 21;269(42):26503-11.
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J Bacteriol. 1995 Apr;177(7):1699-702. doi: 10.1128/jb.177.7.1699-1702.1995.
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