Beneyto P, Alonso I, Pérez T M, López L, Fonseca A
Department of Ophthalmology, Severo Ochoa Hospital, Madrid, Spain.
Ophthalmology. 1996 May;103(5):822-6. doi: 10.1016/s0161-6420(96)30609-x.
Lens autofluorescence originates from an accumulation of fluorescent substances such as the tryptophan-derived residues and glycosylated protein aggregations, which are associated with the process of cataractogenesis and lens aging. The purpose of this investigation is to determine whether pregnancy alters the typical constituents of the lens autofluorescence in patients with diabetes and, if so, to what degree this may occur.
Lens autofluorescence was studied with fluorophotometry in 127 eyes of 72 individuals: 23 control subjects, 6 healthy pregnant women, 21 patients with diabetes, and 44 pregnant patients with diabetes.
The autofluorescence values were 311 +/- 130 ng/ml, 253 +/- 40 ng/ml, 378 +/- 110 ng/ml, and 562 +/- 164 Eq ng/ml (Eq ng/ml = Ng/ml equivalent fluorescein) in the four groups, respectively. The difference between the nonpregnant and pregnant patients with diabetes was significant (P < 0.001).
These results suggest that there is an important deterioration in the metabolic state of the lens during gestation in patients with diabetes, as is the case for diabetic retinopathy.
晶状体自发荧光源于荧光物质的积累，如色氨酸衍生残基和糖基化蛋白聚集体，这些与白内障形成过程和晶状体老化有关。本研究的目的是确定妊娠是否会改变糖尿病患者晶状体自发荧光的典型成分，如果会，这种改变的程度如何。
采用荧光光度法对72名个体的127只眼睛进行晶状体自发荧光研究：23名对照受试者、6名健康孕妇、21名糖尿病患者和44名糖尿病孕妇。
四组的自发荧光值分别为311±130 ng/ml、253±40 ng/ml、378±110 ng/ml和562±164 Eq ng/ml（Eq ng/ml = Ng/ml等效荧光素）。非妊娠糖尿病患者和妊娠糖尿病患者之间的差异具有统计学意义（P < 0.001）。
这些结果表明，糖尿病患者在妊娠期间晶状体的代谢状态会发生重要恶化，糖尿病视网膜病变患者也是如此。
