White C A, Cross S M, Kurilla M G, Kerr B M, Schmidt C, Misko I S, Khanna R, Moss D J
Queensland Institute of Medical Research, Bancroft Centre, Brisbane, Australia.
Virology. 1996 May 15;219(2):489-92. doi: 10.1006/viro.1996.0277.
Epstein-Barr virus (EBV) is the aetiological agent of infectious mononucleosis (IM) which is a common sequel to primary EBV infection. Thereafter, the virus is maintained as a lifetime latent infection. Although the proteins expressed during the latent EBV infection provide a rich source of immunogenic epitopes, very little is known about cytotoxic T lymphocyte (CTL) control of primary EBV infection. The present report is based on an analysis of CTL clones derived from a patient suffering from acute IM. An intriguing feature of six CTL clones that displayed an HLA-restricted pattern of cell lysis was their initial coexpression of the T cell markers CD3, CD4, and CD8. Detailed analysis of one of these clones, which was restricted through the class II MHC antigen DR2, revealed reactivity with an epitope within the EBV lytic cycle early antigen, BHRF-1, which corresponds to the C-terminal region of the protein (AGLTLSLLVICSYLFISRG) (residues 171-189). There have been no previously published reports describing a CTL response during acute IM directed against an EBV lytic antigen. Interestingly, the coexpression of CD4 and CD8 by these CTLs during acute IM suggests that CD3+CD4+CD8+ cortical thymocytic precursor cells are recruited in order to overcome the EBV infection.
爱泼斯坦-巴尔病毒（EBV）是传染性单核细胞增多症（IM）的病原体，IM是原发性EBV感染常见的后续病症。此后，该病毒以终身潜伏感染的形式持续存在。尽管潜伏性EBV感染期间表达的蛋白质提供了丰富的免疫原性表位来源，但对于细胞毒性T淋巴细胞（CTL）对原发性EBV感染的控制了解甚少。本报告基于对一名急性IM患者来源的CTL克隆的分析。六个呈现HLA限制性细胞裂解模式的CTL克隆的一个有趣特征是它们最初共同表达T细胞标志物CD3、CD4和CD8。对其中一个通过II类MHC抗原DR2限制的克隆进行详细分析，发现其与EBV裂解周期早期抗原BHRF-1内的一个表位发生反应，该表位对应于该蛋白质的C末端区域（AGLTLSLLVICSYLFISRG）（第171 - 189位氨基酸）。此前尚无关于急性IM期间针对EBV裂解抗原的CTL反应的报道。有趣的是，这些CTL在急性IM期间CD4和CD8的共同表达表明，CD3 + CD4 + CD8 + 皮质胸腺细胞前体细胞被募集以克服EBV感染。
